Abstract Filamentous fungi are crucial for recycling of organic material in nature. In natural habitats, they cope with many stress factors and therefore their adaptation ability to various conditions is very high. Trichoderma sp., used agriculture as biocontrol agent, exposed a variety toxic molecules including pesticides fungicides. They have fight using mechanisms known the response. Adaptat...

The gene encoding chitinase from Streptomyces sp. (strain J-13-3) was cloned and its nucleotide structure was analyzed. The chitinase consisted of 298 amino acids containing a signal peptides (29 amino acids) and a mature protein (269 amino acids), and had calculated molecular mass of 31,081Da. The calculated molecular mass (28,229Da) of the mature protein was almost same as that of the native ...

BACKGROUND AND OBJECTIVES Mortality is highly variable within population of cultured fish due to virulent bacteria causing fish septicemia. The use of nano-silver marine fungal chitosan as antibiotic synergisers could be an alternative in the treatment of sepsis fish pathogens. MATERIALS AND METHODS Different bulk chitosan solutions were prepared from the mycelia of four marine fungi (Aspergi...

To investigate structure-function relationships in plant chitinases, we have developed a heterologous expression system for the 26 kDa endochitinase from Hordeum vulgare L. (barley). Escherichia coli cells harbouring the gene in a T7 RNA polymerase-based expression vector synthesized completely insoluble recombinant protein under standard induction conditions at 37 degrees C. However, a concent...