The eukaryotic tRNA:guanine transglycosylase (TGT) catalyses the base-for-base exchange of guanine for queuine (the q-base)--a nutrition factor for eukaryotes--at position 34 of the anticodon of tRNAsGUN (where 'N' represents one of the four canonical tRNA nucleosides), yielding the modified tRNA nucleoside queuosine (Q). This unique tRNA modification process was investigated in HeLa cells grow...

A team from the Naval Postgraduate School conducted a trade-off analysis of in-sourcing (i.e., make) versus outsourcing (i.e., buy) the production of legacy parts for ship maintenance and modernization with a focus on cost savings given the need for cost-effective sustainment. The purpose of the study was to compare the make/buy trade-off for a comparison of implementing 3DLST, 3DP, and CPLM fo...

Self-complementary chimeric oligonucleotides (COs) composed of DNA and modified RNA residues were evaluated as a means to (i) create stable, site-specific base substitutions in a nuclear gene and (ii) introduce a frameshift in a nuclear transgene in plant cells. To demonstrate the creation of allele-specific mutations in a member of a gene family, COs were designed to target the codon for Pro-1...

A sodium dodecyl sulfate modified carbon paste electrode (SDS/CPE) was developed in this work based on the surface modification method. The modified electrode was prepared by immersing SDS/CPE in a nickel nitrate solution. This electrode showed a strong accumulation ability toward nickel ions. Then modified electrode was conditioned by potential recycling in a potential range of 0.2–0.8 V (vs. ...

This study was conducted to investigate the effect of tofu wastewater (TW) addition to the growth medium on the growth of Spirulina platensis. The TW addition was varied in range of 0 – 8 %v/v. The results showed that the growth rate (μ) of S. platensis at TW addition of 0, 2, 4, 6, 8 %v/v was 0.007, 0.084, 0.074, 0.088, 0.086 mg/L/d, respectively. The medium with TW content of 6 %v/v (carbon:n...

This study compared the use of Diamond's modified medium to InPouch for the culture of Trichomonas vaginalis from pooled vaginal secretions. The sensitivity for InPouch was 82.4% (61/74) versus 87.8% (65/74) for Diamond's modified medium. There were no significant differences in the sensitivity and negative predictive value of InPouch compared to Diamond's modified medium.

Application of a delaminated kaolin clay base coat onto linerboard provides a barrier coating for a subsequently applied polymer top coat with potential advantages of increased paper stiffness, improved water resistance at reduced polymer overcoat. Motivated by coating material cost reduction, feasibility of the technique is demonstrated by applying a delaminated clay coating online followed by...

Keratinocytes are the main cell type of the epidermis. They secrete a variety of proteins and peptides that have diverse roles in epidermal physiology. In this report, we present purification and partial amino acid sequence of LEKTI, a serine proteinase inhibitor, and DAN (NO3) zinc-finger protein, a tumor suppressor protein of neuroblastoma, from human keratinocyte conditioned medium. Epiderma...

Leaves from in vitro proliferating shoots of cvs. Chandler and Hartley of Persian walnut were cultured on MS and NN media supplemented with BA and NAA. Three light conditions including darkness, low light intensity and modified light intensity were used. Most of the calli were friable and non-embryonic in all three conditions. Rate of callogenesis depended on cultivar, culture medium, light con...