background: during the last twenty years, the extraction of specific egg yolk (igy) antibodies from the immunized chickens has been accepted as a useful alternative to the immunization of mammals. the aim of the present study was immunizing the chickens with human umbilical cord serum (hucs) and the extraction of specific anti-human globulins (igg, c3b, and c3d) antibodies from egg yolk in orde...

deficiency. It is not applicable to newborn or mass screening with use of ifiterpaper specimens, but is satisfactory for liquid urine screening. In one variant of sulfite oxidase deficiency, due to a defect of its molybdate cofactor metabolism, the concentration of uric acid in serum is decreased. Thus, an unexplained low serum uric acid, especially in a young patient with neurologic abnormalit...

Recombination systems based on lambda and Cre/loxP have been described to facilitate gene transfer from one vector to another in a high-throughput fashion, avoiding the bottlenecks associated with traditional cloning. However, no system described to date is suitable for the cloning of affinity reagents selected from display libraries, which requires that the recombination signals flanking the a...

We developed a new approach for chemical ligation of oligonucleotides using the electrophilic phosphorothioester (EPT) group. A nucleophilic phosphorothioate group on oligonucleotides was converted into the EPT group by treatment with Sanger's reagent (1-fluoro-2,4-dinitrobenzene). EPT oligonucleotides can be isolated, stored frozen, and used for the ligation reaction. The reaction of the EPT o...

THE POOR QUALITY of reagent chemicals in the United States by 1916 led to the statement by Hillebrand (1) that “the situation is particularly deplorable just now.” In 1919, to overcome the handicap of poor reagent chemicals, representatives of iiidustry, together with representatives of reagent users, undertook jointly under the sponsorship of the American Chemical Society, the task of establis...

background fenton (fe2+ and h2o2) and modified fenton (fe3+ and h2o2) are two popular methods used in advanced oxidation processes (aop) and degradation of persistent organic pollutants (pops), such as dye compounds. in these processes, fe2+ and fe3+ as catalysts and h2o2 as the oxidizing agent are added to the reactor. objectives the aim of the current study is to assess the abovementioned met...

the heterogeneous photo-fenton degradation of crystal violet under visible light has been investigated usingcopper modified iron oxide. the photocatalyst has been prepared by coprecipitation method. the rate ofphotocatalyic degradation of dye was monitored spectrophotometrically. it has been observed thatphotocatalytic degradation follows pseudo first order kinetics. the effect of various param...

CENTA is a newly synthesized, beta-lactamase-labile, chromogenic cephalosporin reagent which changes color from light yellow (K maximum ca. 340 nm) to chrome yellow (A maximum ca. 405 nm) concomitant with hydrolysis of the betalactam ring. This compound offers promise as a diagnostic reagent comparable to other chromogens (PADAC and nitrocefin) for the early detection of betalactamase-producing...