1. Apparently all disulphide bridges of transferrin and conalbumin were broken by reduction-alkylation, whereas sulphitolysis resulted in incomplete cleavage of disulphide bonds. 2. The molecular weights of reduced-alkylated and sulphitolysed transferrin and reduced-alkylated conalbumin were identical with those of native proteins in a number of solvents, indicating that these proteins exist as...

A L T H O U G H numerous red cell antigens, two electrophoretic forms of hemoglobin and at least 13 electrophoi*etic forms of transferrin have been described in domestic sheep (see RASMUSEN 1962; OGDEN 1961 ; and ASHTON and F E R G U ~ O N 1963), there have been no reports in which these three kinds of markers have been used conjointly in breed comparisons. Furthermore, there have been no repor...

Seven type 1 field isolates of Pasteurella haemolytica were screened for their ability to use different transferrins as a source of iron for growth. All seven strains were capable of using bovine but not human, porcine, avian, or equine transferrin. A screening assay failed to detect siderophore production in any of the strains tested. Iron-deficient cells from these strains expressed a binding...

Transferrins bind ferric ion and deliver the iron to cells. The mechanism of the iron release has been studied kinetically, in vitro, with the aid of single point mutants in which the iron-binding ligand, Asp63 (aspartic acid-63, D63), has been changed to Ser, Asn, Glu and Ala. Iron release from the unmutated N-lobe of human serum transferrin (hTF/2N) by EDTA is influenced by a variety of facto...

Few studies of genetically determin ed traits, other than erythrocyte antigens, or phenylthiocarbamide taste ability (1 , ~, .1,r" IO), have been conducted in leprosy, Therefore, as part of an investiga tion of genetic polymorphism and leprosy conducted under the auspices of the Leonard Wood Memorial, five genetic markers were studied in leprosy patients and controls from Cebu, Philippines. Inc...

Strains of Pseudomonas aeruginosa able to grow readily in serum (serum resistant) produce siderophores in large quantity, enabling them to extract iron from transferrins. The term pyochelin has been proposed for this group of compounds. Pyochelin extractable with ethyl acetate and designated pyochelin A appears to be a mixture of catechols and other phenolates. The structures of water-soluble s...

Polyacrylamide-gel electrophoresis in urea was used to prepare the four molecular species of transferrin:diferric transferrin, apotransferrin and the two monoferric transferrins with either the C-terminal or the N-terminal metal-binding site occupied. The interaction of these 1251-labelled proteins with rabbit reticulocytes was investigated. At 4°C the average value for the association constant...

Haemophilus somnus strain 649 was found to acquire iron from ovine, bovine, and goat transferrins (Tfs). Expression of Tf receptors, as evaluated by solid-phase binding assays, required the organisms to be grown under iron-restricted conditions in the presence of Tf. Competition binding assays revealed the presence of two distinct Tf-binding receptor systems, one specific for bovine Tf and the ...

Congenital disorders of glycosylation (CDG) form a group of autosomal recessive metabolic disorders arising from defects occurring during the biosynthesis of protein glycans. There have been extensive studies into N-linked glycan disorders in which transferrins are glycosylated to different degrees. There have been far fewer studies on the O-linked disorders which predominantly involve errors i...