نتایج جستجو برای: transcripts
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Purpose: The aim of this study was to assay the profile of MCT1 & MCD in mouse unfertilized & fertilized oocytes and preimplantation embryos In vivo and In vitro. Materials and Methods: The presence of mRNAs encoding MCT1 & MCD3 were determined On unfertilized and fertilized oocytes, 2-cell, morulae, blastocyst and cultured embryos in plus glucose KSOM, minus glucose KSOM and pulse glucose KSO...
Two sequences (ARS18 and ARS68) displaying autonomous replication activity were previously cloned in the yeast Yarrowia lipolytica. The smallest fragment (1-1.3 kb) required for extrachromosomal replication of a plasmid is significantly larger in Y. lipolytica than in Saccharomyces cerevisiae. Neither autonomously replicating sequence (ARS) is homologous with known ARS or centromere (CEN) conse...
Recently many exciting advances have been achieved in our understanding of Drosophila meiosis due to combined cytological and genetic approaches. New techniques have permitted the characterization of chromosome position and spindle formation in female meiosis I. The proteins encoded by the nod and ncd genes, two genes known to be needed for the proper partitioning of chromosomes lacking exchang...
Isoforms of importin-α have been identified in insect and human cells, and cross-linking experiments suggest that at least one isoform in each species participates in the targeting of integral membrane proteins to the inner nuclear membrane (INM). To directly test this hypothesis, an assay was developed using Saccharomyces cerevisiae. The data show that internal promoters are present within KAP...
introduction to evaluate the expression patterns of cytokeratin (k) 12, 13, and 19 in normal epithelium of the human ocular surface to determine whether k13 could be used as a marker for conjunctival epithelium. methods: total rna was isolated from the human conjunctiva and central cornea. those transcripts that had threefolds or higher expression levels in the conjunctiva than the cornea were ...
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