The N6,02’-dibutyryl analogue of cyclic adenosine 3’,5’monophosphate (dibutyryl cyclic AMP) elevates the level of hepatic tyrosine-cr-ketoglutarate transaminase (L-tyrosine: 2-oxoglutarate aminotransferase, EC 2.6.1.5) some 3to 6-fold in intact and adrenalectomized rats. The response of this enzyme is more rapid to dibutyryl cyclic AMP than to any other inducer of the transaminase. Hourly injec...

Senescence-associated genes (SAGs) are up-regulated during plant senescence and may have been implicated in encoding enzymes involved in metabolism of senescence tissues. The gene Osl2 (GABA:pyruvate-transaminase) which is upregulated during leaf senescence in rice (Oryza sativa) was functionally characterized. The recombinant Osl2 fusion protein over-expressed in Escherichia coli displays pyru...

We have isolated and characterized an aspartate transaminase (glutamate:oxalacetate transaminase, EC 2.6.1.1) from the thermophilic microorganism Bacillus stearothermophilus. The purified enzyme has a molecular mass of 40.5 kDa by sodium dodecyl sulfate gel analysis, a temperature optimum of 95 degrees C, and a pH optimum of 8.0. The corresponding gene, aspC, was cloned and overexpressed in Esc...

OBJECTIVES This study was undertaken to determine the effect of subacute exposure of peracetic acid on lipid peroxidation and hepatic enzymes in Wistar rats. METHODS 48 male animals in Treatment Group I, II and III received 0.2%, 2% and 20% peracetic acid daily for 2 and 4 weeks. RESULTS Serum malondialdehyde increased and Alanine Transaminase and Aspartate Transaminase decreased significan...

The simultaneous application of chemical modification of primary amines and the insertion of a lgF probe have been used to detect the nature and physical properties of the amino group influencing a selected region of the active center of the enzyme aspartate aminotransferase. The enzyme, carbamylated at the NH,-terminal alanine (transaminase’, produced from cyanate-treated holoenzyme) or at the...

Protective efficacy of DL-alpha lipoic acid on adriamycin induced hepatotoxicity was evaluated in rats. Adriamycin toxicity, induced by a single injection (ip; 15 mg/kg body wt), was expressed by an elevation in alanine transaminase, aspartate transaminase, bilirubin levels in serum and alkaline phosphatase, lactate dehydrogenase, alanine transaminase, aspartate transaminase activity in hepatic...

The effects of cortisone on the dietary induction and repression of serine dehydrase, ornithine transaminase, and tyrosine transaminase were studied. In rats pretreated with cortisone dietary induction was affected relatively little, but carbohydrate repression was largely eliminated. The effects of cortisone and phenobarbital on the dietary induction of serine dehydrase and ornithine transamin...

Glycogenic hepatopathy (GH) is an uncommon cause of serum transaminase elevation in type I diabetes mellitus (DM). The clinical signs and symptoms of GH are nonspecific, and include abdominal discomfort, mild hepatomegaly, and transaminase elevation. In this report we describe three cases of patients presenting serum transaminase elevation and hepatomegaly with a history of poorly controlled ty...

The effects of cortisone on the dietary induction and repression of serine dehydrase, ornithine transaminase, and tyrosine transaminase were studied. In rats pretreated with cortisone dietary induction was affected relatively little, but carbohydrate repression was largely eliminated. The effects of cortisone and phenobarbital on the dietary induction of serine dehydrase and ornithine transamin...