The recombinant antibodies RB712 and RB713 detect by ELISA the Drosophila melanogaster Spargel protein (or GCH) fused to a GST protein.

Since glucose biosensors are one of the most popular and widely used point-of-care testing devices, a novel electrochemical enzyme-linked immunosorbent assay (ELISA) for protein biomarkers has been developed based on a glucose detection strategy. In this study, α-fetoprotein (AFP) was used as the target protein. An electrochemical ELISA system was constructed using anti-AFP antibodies immobiliz...

Background: SLC39A6 Protein (solute carrier family 39) or LIV-1 is a zinc transporter protein that is overexpressed in positive estrogen cancers such as breast cancer. The LIV-1 protein transfer zinc into the cytoplasm through the plasma membrane. Today it is known that just as a decrease in the concentration of zinc in the cell can cause cancer, an excessive increase in the concentration of zi...

A competitive ELISA for the determination of protein-A concentration in culture supernatants is described. The sensitivity of the assay is 50 ng/ml and it may be used to determine concentrations of protein-A up to 1000 ng/ml. The assay is specific, rapid and suitable for routine screening of protein-A producing microorganisms.

An enzyme-linked immunosorbent assay (ELISA) is described for detection of anti-capsular antibodies against Haemophilus influenzae type b. Polyribosephosphate was covalently bonded to poly-L-lysine before adsorption to microtiter plates. ELISA immunoglobulin G and immunoglobulin M anti-polyribosephosphate antibody titers were comparable to total anti-polyribosephosphate antibody concentration d...

  The effects of gentamicin, lithium and combination of these drugs on ultrastructural morphology of rat submandibular glands were studied. Daily intraperitoneal injection of 40 mg/kg of gentamicin which causes chronic salivary gland toxicity, led to a marked decrease in gland's dry weight and total protein concentration but did not affect DNA content. Electron micrograph (EM) of submandibular ...

Background: The aim of present study was to estimate the levels of some Trace element (Lead, Copper, Cobalt, Chromium, and Cademium) concentrations in Asthenospermia and Normospermia by atomic absorption method, in comparison with control (Fertile), Estimate concentration of (MDA) Malondialdehyde and Total protein concentration in semen specimens.Also relationships between mentioned components ...

Due to our interest in the chemical space of organic dyes identify potential small-molecule inhibitors (SMIs) for protein-protein interactions (PPIs), we initiated a screen such compounds assess their inhibitory activity against interaction between SARS-CoV-2 spike protein and its cognate receptor ACE2, which is first critical step initiating viral attachment entry this coronavirus responsible ...

A night soil treatment plant which collects a high amount of human urine and excreta, is a very unique system to Japan and the object of this investigation on the fate and behavior of the human estrogens in the water treatment process. Water and sludge samples at different treatment phases were taken at a night soil treatment plant. Their hydrophobic fractions were assayed using an in vitro yea...

The aim of the present study was to investigate the impact of whey protein coating on quality and shelf life of Kilka fish. Whey protein edible coating was prepared in three different concentrations, including 3, 7, 10 and 13%. Then gutted Kilkas were coated for 1h, packed in polyethylene dishes with cellophane blanket and stored at -18 °C. Total microbial count, total volatile nitrogen (TVN) a...