CD4+ tissue-resident memory T (TRM) cells persist in the skin after an allergic reaction and induce rapid inflammation upon antigen re-exposure. However, mechanisms underlying maintenance of TRM their functional heterogeneity remain largely unknown. To clarify this, we first used a delayed-type hypersensitivity model with adaptive transfer ovalbumin (OVA)-specific using cell receptor-β deficien...

Abstract Background p63, a member of the p53 gene family, is an important regulator for epithelial tissue growth and development. ?Np63? main isoform p63 highly expressed in Non-melanoma skin cancer (NMSC). Extracellular signal-regulated kinase 3 (ERK3) atypical mitogen-activated protein (MAPK) whose biochemical features cellular regulation are distinct from those conventional MAPKs such as ERK...

INTRODUCTION: Certain mineral elements dismetabolism is known to play an important part in the pathogenesis of corneal degenerative and dystrophic diseases, as metalloenzymes participate connective tissues metabolism affecting their properties. Thus, keratoconus tissue depleted iron, copper zinc, what could be underlying cause cornea biomechanical properties impairment. Keratoconus modeling com...

In both Drosophila melanogaster and mammalian systems, epithelial structure and underlying cell polarity are essential for proper tissue morphogenesis and organ growth. Cell polarity interfaces with multiple cellular processes that are regulated by the phosphorylation status of large protein networks. To gain insight into the molecular mechanisms that coordinate cell polarity with tissue growth...

Ischemia-reperfusion (IR) injury causes a vigorous immune response that is amplified by complement activation, leading to local and remote tissue damage. Using MRL/lpr mice, which are known to experience accelerated tissue damage after mesenteric IR injury, we sought to evaluate whether complement inhibition mitigates organ damage. We found that complement depletion with cobra venom factor prot...

We report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDRNA removes 98% of targeted RNAs while preserving relative abundance profiles of non-targeted RNAs...

STUDY OBJECTIVE Neutrophils are involved in acute lung injury during ARDS via several mechanisms. This study focuses on neutrophil-derived oxidative stress. Hypochlorite is a major neutrophil-derived oxidant. This study characterizes hypochlorite-induced acute changes in pulmonary circulation and the involvement of tissue lipid peroxidation (LPO) and reduced glutathione (rGSH) depletion. METH...

The purpose of the present study is to examine the effects of ego depletion on arousal of elite Iranian swimmers. The sample comprised elite swimmers invited to the national swimming team as focused and semi focused (36 subjects). The research design was as pre- and post-test—in two phases of ego depletion conditions and the lack of ego depletion conditions in two experimental and control...

Neutrophils are thought to play an important role during contact hypersensitivity (CHS) in mice, a notion which is supported by studies in which neutrophils are depleted by monoclonal antibodies (mAb). Here, we show that administration of the commonly used anti-mouse Ly6G/C mAb (clone RB6.8C5) leads to depletion of not only neutrophils but also a population of monocytes and macrophages. In cont...