Vitrification of endothelial cells (MHECT-5) has not previously been compared with controlled slow freezing methods under standardized conditions. To identify the best cryopreservation technique, we evaluated vitrification and standardized controlled-rate -1°C/minute cell freezing in a -80°C freezer and tested four cryoprotective agents (CPA), namely dimethyl sulfoxide (DMSO), ethylene glycol (...

the potential mycoherbicide alternaria alternata was isolated from one lesion on a leaf of amaranthus retroflexus which had been inoculated with conidia of the fungus. the isolate was cultured on v-8 agar medium. hyphal tip isolates (hti) were derived from young germ tubes of single spores and cultured on a semidefined culture medium with a c:n ratio of 15:1. amplified fragment length polymorph...

The aim of the present investigation was to standardize in vitro shoot tip culture technique considering various aspects for direct plant formation two onion (Allium cepa L.) varieties ‘Indian’ and ‘Taherpuri’. About 1.5-2.2 mm tips were collected from bulbs sterilized with 0.1% HgCl2 duration. Then they cultured on MS medium supplemented hormonal concentrations different media composition prim...

Catheter-associated bacteremia (CAB) is a frequent cause of nosocomial infection in the critical patient and implies an increase in both morbidity--mortality and healthcare costs. The classical method for confirming CAB involves the concomitant isolation of the microorganism in blood cultures obtained by percutaneous puncture and from catheter tip cultures. This conventional procedure has the i...