conclusions torghabeh - shandiz district with a rural texture is endemic for anthroponotic cutaneous leishmaniasis (acl). further studies are recommended to discover the vectors and probable reservoirs in these areas. results of 164 samples obtained from the skin lesions of the patients referred to the torghabe - shandiz health centers, leishmania dna was isolated from 136 smears by pcr method....

conclusions evidence of t. gondii occurrence in sheep and camels was provided in the provinces under study. there is a need to investigate the potential risk factors of zoonotic infections. furthermore, animal health and production losses caused by the parasite; and associated zoonotic implications in the area under study need to be explored. materials and methods animals in the field and those...

background cholera is a severe diarrheal illness caused by vibrio cholerae, which produces a virulence factor named el tor hemolysin encoded by the hlya gene. objectives this study meant to analyze the phenotypic characteristics and presence of hlya gene in v. cholerae isolated from patients in iran. the hlya gene which codes for hemolysin, plays an essential role in manifestation of cholera ,a...

background brucellosis is the most common global zoonosis and an important public health problem in many parts of the world including iran. diagnosis of brucellosis is frequently difficult to establish and conventional methods are not always successful in identifying the organisms. rapid detection of brucella species by an automated blood culture system and polymerase chain reaction (pcr) may l...

background: some dynamic changes occurs during spermatogenesis such as histone removal and its replacement with transition nuclear protein and protamine. these proteins are required for packing and condensation of sperm chromatin. jhdm2a is a histone demethylase that directly binds to promoter regions of tnp1 and prm1 genes and controls their expression by removing h3k9 at their promoters.objec...

The scientific, medical, and diagnostic communities have been presented the most powerful tool for quantitative nucleic acids analysis: real-time PCR [Bustin, S.A., 2004. A-Z of Quantitative PCR. IUL Press, San Diego, CA]. This new technique is a refinement of the original Polymerase Chain Reaction (PCR) developed by Kary Mullis and coworkers in the mid 80:ies [Saiki, R.K., et al., 1985. Enzyma...

Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents. In this regard, the identification of Brucella can be a time-consuming and labor-intensive process posing a real risk of laboratory-acquired infection to the laboratory staff. This stud...

conclusions it is suggested that determination of the target genotype by plasma subtyping for choosing the proper antiviral therapy is essential but may result in therapy failure. hcv genotyping in pbmc samples, along with plasma specimens, might be more beneficial. therefore determining the hcv genotype in pbmcs, before beginning the therapy is useful due to the possibility of occult infection...