The C cell complexes in the dog thyroid are the remnants of ultimobranchial bodies which retain fetal characteristics, and contain cyst structures showing various sizes, shapes and liminal contents. This study deals with these cysts with regard to their morphology, immunoperoxidase reactions to anti-calcitonin, anti-19S thyroglobulin and anti-C-thyroglobulin antisera, and ability to incorporate...

OBJECTIVE The purpose of the present work is to establish the intron-exon organization from exon 12 to exon 23 of the human thyroglobulin gene and to construct a physical map of the 5' terminal half of the gene. DESIGN Screening of a genomic library and subsequent restriction map, hybridization and sequencing methods have been employed to characterize the recombinant positive phages. METHOD...

When CD4+ CD25+ regulatory T cells are depleted or inactivated for the purpose of enhancing antitumor immunity, the risk of autoimmune disease may be significantly elevated because these regulatory T cells control both antitumor immunity and autoimmunity. To evaluate the relative benefit and risk of modulating CD4+ CD25+ regulatory T cells, we established a new test system to measure simultaneo...

Thyroglobulin was isolated from thyroid glands of normal guinea pigs and from animals treated with thiouracil. These preparations were fractionated by isopyknic centrifugation in RbCl into proteins of varying iodine content. When the disulfide bonds of these protein fractions were reduced and analyzed by polyacrylamide gel electrophoresis in Na dodecyl-SO4, t hree species were observed with mol...

In this sequential assay, the thyroglobulin in serum binds to polystyrene beads coated with two mouse monoclonal antibodies. These beads then react with a rabbit polyclonal antibody, biotinylated sheep anti-rabbit IgG, streptavidin-horseradish peroxidase, and a peroxidase substrate to yield a colored product that is measured spectrophotometrically at 492 nm. The range of the standard curve is 1...

Endemic goiter is clustered occurrences of goiter affecting more than 10% of the population in particular regions. The majority cases are attributed to environmental factors such as iodine deficiency, or food and water containing goiter-causing agents called goitrogens. We report a new type of endemic goiter where the basic abnormality was not due to environment factors, but to thyroglobulin ge...

Thyroglobulin (Tg) is a large glycoprotein of mol.wt. 660000 synthesized in the thyroid. It is the vehicle for the unique route of biological synthesis of thyroxine and contains up to six thyroxyl residues in peptide linkage within its peptide chain. Despite the fact that small quantities are present in the human circulation from birth onwards auto-antibodies to thyroglobulin are commonly found...