RATIONALE Mitochondrial-derived hydrogen peroxide (H2O2) regulates flow-induced dilation (FID) in microvessels from patients with coronary artery disease. The relationship between ceramide, an independent risk factor for coronary artery disease and a known inducer of mitochondrial reactive oxygen species, and FID is unknown. OBJECTIVE We examined the hypothesis that exogenous ceramide induces...

BACKGROUND Urinary organic acids are water-soluble intermediates and end products of the metabolism of amino acids, carbohydrates, lipids, and a number of other metabolic processes. In the hereditary diseases known as organic acidurias, an enzyme or co-factor defect in a metabolic pathway leads to the accumulation and increased excretion of one or more of these acidic metabolites. Gas chromatog...

The Single Point Ramped Imaging with T1 Enhancement (SPRITE) sequence is well suited for the acquisition of magnetic resonance signals from fast relaxing nuclei and from heterogeneous materials. However, it is time inefficient compared to sequences that are based on frequency encoding because only one single point is acquired per excitation. Multiple-point SPRITE (mSPRITE) mitigates this proble...

a very simple, rapid and sensitive dispersive liquid-liquid microextraction (dllme) followed by gas chromatography and flame ionization detection (gc-fid) was developed for the determination of phenol and carvacrol in honey samples. a mixture of 100 µl dichloromethane (extraction solvent) and 0.5 ml acetonitrile (disperser solvent) was rapidly injected into sample solution. thereby a cloudy sol...

A dimeric neomycin-neomycin conjugate 3 with a flexible linker, 2,2'-(ethylenedioxy)bis(ethylamine), has been synthesized and characterized. Dimer 3 can selectively bind to AT-rich DNA duplexes with high affinity. Biophysical studies have been performed between 3 and different nucleic acids with varying base composition and conformation by using ITC (isothermal calorimetry), CD (circular dichro...

Comprehensive two-dimensional gas chromatography (GCxGC) offers an interesting tool for profiling bacterial fatty acids. Flow modulated GCxGC using a commercially available system was evaluated, different parameters such as column flows and modulation time were optimized. The method was tested on bacterial fatty acid methyl esters (BAMEs) from Stenotrophomonas maltophilia LMG 958T by using para...

Radiology & Biomedical Imaging, University of California San Francisco, San Francisco, CA, USA Full list of author information is available at the end of the article Figure 1 Navigator signal (left) and T2-weighted TSE image (right) for reference (top), non-gated breathing (middle), and gated breathing (bottom) scans. The dashed line in the bottom left panel represents the gating threshold. Dyv...

Mineral oil (or Hydrocarbon oil) in water samples is determined by liquid-liquid extraction, followed by a clean-up step on Florisil, and GC-FID analysis. Using the Agilent 7696A Sample Prep WorkBench system, drying and clean-up of samples obtained after liquid-liquid extraction can be automated. High recoveries and excellent reproducibility are obtained for the mineral oil fraction, while the ...

Hydrocarbons from samples of traffic-polluted urban air were separated by gas chromatography on an aluminium oxide column and assessed simultaneously by photoionization detection (PID) and flame ionization detection (FID) after effluent splitting. The 10.2 eV photoionization detector selectively detects alkadienes and alkenes but not alkanes and alkynes in the C3-C5 region. The maximum PID/FID ...