Submit Manuscript | http://medcraveonline.com Abbreviations: HR: Hypersensitive Response; SAR: Systemic Acquired Resistance; WIPK: Wound-Induced Protein Kinase; SIPK: Salicylic Acid-Induced Protein Kinase; TCA: Trichloroacetic Acid; 2-ME: 2-Mercaptoethanol; SDS: Sodium Dodecyl Sulphate; PMSF: Phenyl Methyl Sulfonyl Fluoride; 2DE: 2D Gel Electrophoresis; DTT: Dithiothreitol; aact: Acetyl-CoA C-A...

We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membranes is quantified using four types of well-established biological tests: a trypan blue solution, the tetrazolium salt WST-1 reagent, the LDH c...

Cytotoxicity testing of aflatoxin (AF) on the viability of cells grown in cultures can be widely used to predict the potential toxic effects of AF in animals. To this end, an in vitroexperimental study was conducted to ascertain the toxic effects of AF extracts obtained from compound feeds in South Africa on human lymphocytes in comparison to that of an AFB1 standard. The approach adopted was o...

With the increased use of tetrazolium salts in biological systems (l-20), and with the increased availability of tetrazolium salts with widely different oxidation-reduction potentials (3-5, 11, 14, 32), differences in the effect of anaerobiosis on the reaction rate (3, 6, 9, 10, 16, lQ), in the effect of disrupting influences such as freeze-thawing or section thinness (6, 10, 16, lQ), and in th...

The nitro blue tetrazolium test was applied on neutrophils from children with kwashiorkor and from control children. The percentage of formazan cells formed in the kwashiorkor patients was significantly less than in the control group in spite of the presence of associated infections in the former. A direct correlation exists between the percentage of formazan cell formation and total serum prot...

A mouse fibrosarcoma cell line (FSa-II), which exhibits low endogenous levels of manganese Superoxide dismutase, was transfected with a human manganese Superoxide dismutase complementary DNA. Fifty clones were screened for manganese Superoxide dismutase activity by the Superoxide dismutase activity gel assay. Activity of the positive clones was measured by the nitro blue tetrazolium-reduction a...

BACKGROUND Blue light is a high-energy or short-wavelength visible light, which induces retinal diseases such as age-related macular degeneration and retinitis pigmentosa. Bilberry (Vaccinium myrtillus L.) and lingonberry (Vaccinium vitis-idaea) contain high amounts of polyphenols (anthocyanins, resveratrol, and proanthocyanidins) and thus confer health benefits. This study aimed to determine t...

The synthesis of salts of the type R„SH3_„+MF6~ (R = C2H5, i-C3H7; n = 1, 2; M = As, Sb) by protonation of the corresponding thiols and sulfides in the superacid systems HF/MF5 is reported. The salts have been characterized by vibrational and NMR spectroscopic methods. Isopropylsulfonium hexafluoroantimonate is the first known example of a sulfonium salt, for which a SH bond distance has been d...