نتایج جستجو برای: thermus aquaticus

تعداد نتایج: 2175  

Journal: :Frontiers in microbiology 2016
Yuko Nagayoshi Kenta Kumagae Kazuki Mori Kosuke Tashiro Ayano Nakamura Yasuhiro Fujino Yasuaki Hiromasa Takeo Iwamoto Satoru Kuhara Toshihisa Ohshima Katsumi Doi

A filamentous bacteriophage, φOH3, was isolated from hot spring sediment in Obama hot spring in Japan with the hyperthermophilic bacterium Thermus thermophilus HB8 as its host. Phage φOH3, which was classified into the Inoviridae family, consists of a flexible filamentous particle 830 nm long and 8 nm wide. φOH3 was stable at temperatures ranging from 70 to 90°C and at pHs ranging from 6 to 9. ...

2007
Scott Bailey William K. Eliason Thomas A. Steitz

The ring-shaped hexameric DnaB helicase unwinds duplex DNA at the replication fork of eubacteria. We have solved the crystal structure of the full-length Thermus aquaticus DnaB monomer, or possibly dimer, at 2.9 A resolution. DnaB is a highly flexible two domain protein. The C-terminal domain exhibits a RecA-like core fold and contains all the conserved sequence motifs that are characteristic o...

Journal: :Acta biochimica Polonica 2007
Paweł Filipkowski Józef Kur

To study the biochemical properties of SSB's from Deinococcus grandis (DgrSSB) and Deinococcus proteolyticus (DprSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The genes consist of an open reading frame of 891 (DgrSSB) and 876 (DprSSB) nucleotides encoding proteins of 296 and 291 amino acids with a calculated molecular mass of 32.2...

Journal: :The Journal of biological chemistry 2003
Eric Alani Jae Young Lee Mark J Schofield Amanda W Kijas Peggy Hsieh Wei Yang

During mismatch repair ATP binding and hydrolysis activities by the MutS family proteins are important for both mismatch recognition and for transducing mismatch recognition signals to downstream repair factors. Despite intensive efforts, a MutS.ATP.DNA complex has eluded crystallographic analysis. Searching for ATP analogs that strongly bound to Thermus aquaticus (Taq) MutS, we found that ADP....

Journal: :Applied and environmental microbiology 1988
R J Sharp R A Williams

Seventeen pink-pigmented strains of the genus Thermus were isolated from samples collected from thermal areas of Iceland. The strains were examined by using phenotypic characterization and DNA:DNA homology and were compared with recognized strains. Visually, the strains could be divided into three groups based on their pigmentation; however, spectroscopic studies of the pigments indicated littl...

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