The soil column samples were collected for indoor simulated freeze-thaw experiments to monitor the hydrothermal dynamics and measure basic physicochemical properties research effects of on process peat bog its relationship with properties. results indicate that in initial phase freezing-thawing, water content decreases temperature changes, respectively. Unfrozen stable freezing period sharply. ...

The present study was conducted to observe the effect of osmolality of glycerolated TEST-yolk glycerol extenders on post-thawing sperm kinematics of ram spermatozoa of the native Malpura breed maintained in a semi-arid tropical environment. Good quality semen obtained from adult rams was pooled, split and diluted to 1,000 million spermatozoa per ml in complete TEST-yolkglycerol extenders of 900...

This paper reviews new developments in methods of freezing (high-pressure freezing, dehydrofreezing and applications of antifreeze protein and ice nucleation protein) and thawing (high-pressure and microwave thawing, ohmic thawing and acoustic thawing) for foods. With a good understanding of the solid–liquid phase diagram of water, the effects of pressure on food freezing– thawing cycles are hi...

This study aimed to investigate the effect of storage at ?3 °C on myofibrillar protein in fast or slow frozen pork. Five pork loins 48 h post-mortem were subjected either (cold metal plate/?80 °C) freezing (still air/?20 followed by for 0, 1, 3, and 7 days before thawing. Freezing rate significantly influenced proteins within 3 °C, evidenced higher thaw loss, surface hydrophobicity reduced wate...

The present study was performed to assess the fertility of frozen-thawed dog semen prepared by freezing with 6% glycerol and thawing at 70 degrees C for 8 sec, and to evaluate the least number of post-thaw spermatozoa necessary to achieve pregnancy by intrauterine or intratubal artificial insemination. It was found that the pregnancy rate of intrauterine artificial insemination was 100% using 6...

objective: to evaluate the effect of honey supplemented to cryoprotectant medium on post-thaw sperm motility, concentration, morphology and agglutination. materials and methods: thirty semen samples were collected from 30 infertile patients. after assessment of semen analysis, semen samples were divided into 3 aliquots (0.7ml for each) and mixed with 1 ml of cryopreservation solution (g1, contr...

BACKGROUND Morphometric and morphokinetic evaluation of in vitro cultured human embryos allows evaluation without time restriction and reduces intra- and inter-observer variability. Even though these technologies have been reported to improve the quality of cleavage stage embryo evaluation during fresh culture, possible advantages in the evaluation of cryopreserved embryos have been scarcely ex...

Microbial release of greenhouse gases from thawing permafrost is a global concern. Seventy-six metagenomes were generated from low-soil-organic-carbon mineral cryosols from Axel Heiberg Island, Nunavut, Canada, during a controlled thawing experiment. Permafrost thawing resulted in an increase in anaerobic fermenters and sulfate-reducing bacteria but not methanogens.

The ice crystal formation is assumed as the most lethal factor for the failure of fish embryo cryopreservation and intracellular ice formation (IIF) plays a central role in cell injury during cooling. The objectives were to observe the morphological changes of red seabream (Pagrus major) embryo during the cooling-thawing process, and to examine the effect of cryoprotectant and cooling rate on t...

In this study, we compared the effects of repeated freezing/thawing of human sperm by our in-house method of rapid freezing with slow programmable freezing. Sperm samples from 11 normozoospermic subjects were processed through density gradients and divided into three aliquots: non-frozen, rapid freezing and slow programmable freezing. Sperm in the rapid freezing group had better motility and vi...