A pore-forming protein with an Mr of 40,000 has been extracted from the cell wall of Mycobacterium smegmatis with buffer containing the detergent Zwittergent 3-12 and 0.5 M NaCl and purified on an anion-exchange column. Although the pore diameter was large (2 nm), the specific activity was much lower than those of nonspecific porin channels of enteric bacteria. The channel allowed the permeatio...

alpha-L-fucose abolishes the activity of guinea pig migration inhibitory factor (MIF) on the macrophages. Other sugars such as alpha-D-glucose, beta-D-galactose, alpha-L-rhamnose, methyl-alpha-D-mannoside, and N-acetyl-beta-D-glucosamine had no effect. Theabolition of MIF activity by alpha-L-fucose was reversible. When macrophages were incubated with alpha-L-fucosidase, a glycosidase which spli...

Inhibition studies on the concanavalin A-dextran interaction have been extended using as inhibitors the members of the maltose-, isomaltose-, and methyl cy-isomaltoside series, amino and N-acetyl amino sugars, and a number of oligosaccharides containing more than one linkage type. The interaction of the plant protein at the terminal chain ends of the a-linked glucans and mannans has been essent...

We report development of a method for the direct measurement of the interaction between the N-terminal arm and the remainder of the dimerization domain in the Escherichia coli AraC protein, the regulator of the l-arabinose operon. The interaction was measured using surface plasmon resonance to monitor the association between the immobilized peptide arm and the dimerization domain, truncated of ...

• Overexpression of AtPAP2, a purple acid phosphatase (PAP) with a unique C-terminal hydrophobic motif in Arabidopsis, resulted in earlier bolting and a higher seed yield. Metabolite analysis showed that the shoots of AtPAP2 overexpression lines contained higher levels of sugars and tricarboxylic acid (TCA) metabolites. Enzyme assays showed that sucrose phosphate synthase (SPS) activity was sig...

optimization of the isolation methods and utilization of the sugars present in the hydrolysates obtained from pre-hydrolyses of various lignocellulosic materials for production of new chemicals and energy is currently accounted for novel methods of biorefinery all over the world. in this study, effect of temperature (90 and 100 oc), koh concentration (2, 4 and 6 %) and polyoxyethylene (80) sorb...

in order to investigate the effect of temperature and time of blanching process on the generation and extraction of reducing sugars and kinetics of color changes during blanching, the potato strips packaged in the polyethylene (hdpe with 10 micron thickness) film or without polyethylene packaging, were immersed in distilled water at temperature range of 50, 60, 70, 80 and 90 ºc for 120 minutes....

Measurement error in self-reported sugars intake may explain the lack of consistency in the epidemiologic evidence on the association between sugars and disease risk. This review describes the development and applications of a biomarker of sugars intake, informs its future use and recommends directions for future research. Recently, 24 h urinary sucrose and fructose were suggested as a predicti...

in order to estimate the reducing sugar in tubers of potato by using a manual glucose determiner, an experiment was conducted in factorial arrangement based on completely randomized design with three replications. in this research reducing sugar of four cultivars of potato – agria, sante, with two methods: enzymatic and a manual glucose determiner. the results picaso and kizer – that maintained...