The amino acid-producing organism Corynebacterium glutamicum cannot utilize glycerol, a stoichiometric by-product of biodiesel production. By heterologous expression of Escherichia coli glycerol utilization genes, C. glutamicum was engineered to grow on glycerol. While expression of the E. coli genes for glycerol kinase (glpK) and glycerol 3-phosphate dehydrogenase (glpD) was sufficient for gro...

1. The possible role of glycerol as a precursor in neonatal gluconeogenesis in the rat was investigated by recording the activities of glycerol kinase and l-glycerol 3-phosphate dehydrogenase in the liver, kidney and other tissues around birth and during the neonatal period. 2. Blood glycerol concentrations in the neonatal rat are high. 3. There is a marked increase after birth in the ability o...

1. Glycerol kinase (EC 2.7.1.30) is shown to catalyse a non-equilibrium reaction in rat liver; and, as it is the first enzyme in the pathway metabolizing glycerol, its properties may be pertinent to the metabolic regulation of glycerol uptake and utilization by this tissue. 2. The properties of hepatic glycerol kinase were studied by using a radiochemical technique to measure the enzyme activit...

Jensen, Michael D. Regional glycerol and free fatty acid metabolism before and after meal ingestion. Am. J. Physiol. 276 (Endocrinol. Metab. 39): E863–E869, 1999.—We measured splanchnic and leg glycerol [and free fatty acid (FFA)] uptake and release in 11 healthy volunteers before and after meal ingestion to assess whether regional FFA-to-glycerol release ratios mirror systemic release ratios. ...

STEELE, R., B. WINKLER, AND N. ALTSZULER. Inhibition by infused glycerol of gluconeogenesis from other precursors. Am, J. Physiol. 22 l(3) : 883-888. 1971 .-Plasma glycerol contributes 20% of the carbon of glucose released to the blood by the liver in the 6to 7-dayfasted dog (shown by use of glycerol-UJ4C). This increases to 63y0 when bulk glycerol infusion increases total glycerol uptake from ...

BERGMAN, E. N. Glycerol furnouer in the nonpregnant and k&tic pregnant sheep. Am. J. Physiol. 215(4): 865-873. 1968.Labeled glycerol was infused intravenously into three groups of sheep: fed, fasted, and twin-pregnant hypoglycemic and ketotic. The turnover time of the free glycerol pool was rapid in all animals (15-l 8 min). Mean glycerol turnover rates were 0.25 mmole/hr per kg3i4 in fed anima...

Although glycerol is the primary carbon source available to halophilic heterotrophic communities, little is known regarding haloarchaeal glycerol metabolism. In this study, a gene encoding a glycerol kinase homolog (glpK; HVO_1541) was deleted from the genome of the haloarchaeon Haloferax volcanii by a markerless knockout strategy. The glpK mutant, KS4, readily grew on yeast extract-peptone com...

background and objectives: preparation and evaluation of nanocomposite films have become prevalent in recent years. depending on the purpose of using the film, and considering the effects and interaction effects of nanomaterial and other modifiers on the film properties, the amounts of nanomaterials and modifiers should be optimized. the effects of montmorillonite (mmt), glycerol (gl), and thei...

introduction: biofilm forming staphylococcus epidermidis is a main causative agent of infections related to medical devices. purification and evaluation of gly-ta polysaccharide from a biofilm-forming s. epidermidis as a putative vaccine candidate were the main goals of the current study. methods: taking advantage of size exclusion chromatography procedure, glycerol teichoic acid (gly-ta) was p...

Escherichia coli and Salmonella typhimurium show positive chemotaxis to glycerol, a chemical previously reported to be a repellent for E. coli. The threshold of the attractant response in both species was 10(-6) M glycerol. Glycerol chemotaxis was energy dependent and coincident with an increase in membrane potential. Metabolism of glycerol was required for chemotaxis, and when lactate was pres...