Expression of foreign proteins in E. coli is normally inhibited by exogenous production of acetate. To overcomethis problem, various strategies have been proposed and tested to reduce the extent of acetate accumulation.Although these strategies can improve the outcome, the implementation of their proposed techniquesis not practical. Because to achieve optimal results, it requi...

This article explores area studies contributions to sociolinguistics by examining Sunni reformers’ use of the Arabic term al-qāida, or a ‘‘pragmatic base.” Material is drawn from an audiocassette collection formerly owned by Usāma Bin Lādı n. Divergent approaches to the qāida suggest that the term functions a base for many forms of spatial, temporal, social, and ethical orientation. Much of the...

materials and methods: in order to achieve this, recombinant plasmid harboring antisense sequences targeting both of pta and acka was assembled, after transfecting to the cells, its effects on the cell growth and acetate accumulation in the minimal media was assessed and compared with the control, the plasmid without antisense cassette, in presence and absence of iptg in escherichia coli bl21 (...

multifunctional nanomaterials showed graet advantages in drug delivery. folic acid (fa) binding protein, a glycosyl phosphatidyl inositol anchored cell surface receptor for folate, is overexpressed in several human tumors, whereas it is highly restricted in normal tissues. therefore, in this study, fa, polyethylene glycol (peg), and fe3o4 nanoparticles multifunctionalized short multiwall carbon...

Generation of gain-of-function transgenic mice by targeting the Rosa26 locus has been established as an alternative to classical transgenic mice produced by pronuclear microinjection. However, targeting transgenes to the endogenous Rosa26 promoter results in moderate ubiquitous expression and is not suitable for high expression levels. Therefore, we now generated a modified Rosa26 (modRosa26) l...

A fully automated and efficient radiosynthesis of a novel interleukin-2 radioconjugate from single FASTLab™ cassette.