Antigene oligonucleotides have the potential to regulate gene expression through site-specific DNA binding. However, in vivo applications have been hindered by inefficient cellular uptake, degradation, and strand displacement. Peptide nucleic acids (PNAs) address several of these problems, as they are resistant to degradation and bind DNA with high affinity. We designed two cationic pyrimidine ...

When a target travels slowly and smoothly along the line of sight of one eye, the eye that is aligned with the target remains stationary while the other eye adducts. The mechanism that is commonly invoked is that commands signaling conjugate pursuit and symmetrical vergence are combined. The two signals are in the same direction in the adducting eye but are in the opposite direction in the stat...

In this paper we consider Metropolis-Hastings Markov chains with absolutely continuous with respect to Lebesgue measure target and proposal distributions. We show that under some very general conditions the sequence of the powers of the conjugate transition operator has a strong limit in a properly defined Hilbert space described for example in Stroock [17]. Then we propose conditions under whi...

Several peptide nucleic acid based artificial nucleases (PNAzymes) are designed to create a bulge in the target RNA, which is a short model of the leukemia related bcr/abl mRNA. The target RNA is cleaved by the PNAzymes with a half-life of down to 11 h (using a 1 : 1 ratio of PNA-conjugate to target) and only upon base-pairing with the substrate. The PNA based systems are also shown to act in a...

Cytokines are key modulators of the immune responses and represent promising therapeutics for a variety cancers. However, successful translation cytokine-based therapy to clinic is limited by, among others, severe toxicities lack efficacy due cytokine pleiotropy off-target activation cells. Engineering cytokines with enhanced therapeutic properties has emerged as strategy overcome these challen...

the efficiency and robustness of reliability methods are two important factors in first order reliability method (form). the conjugate choice control (ccc) and directional chaos control method (dcc) were developed to improve the robustness and efficiency of form formula using the stability transformation method. in this paper, the ccc and dcc methods are applied for reliability analysis of a co...

conclusions the prepared antibody can be used for the development of an elisa kit to assay afm1 in milk and other biological fluids. background aflatoxins are the most extensively studied group of mycotoxins produced by molds, especially the aspergillus group, which are highly toxic to animals and humans. objectives since immunoassay is a simple and rapid method for the analysis of many toxic s...

in this study it has been tried, to compare results and convergence rate of sensitivity analysis and conjugate gradient algorithms to reduce fuel consumption and increasing engine performance by optimizing the timing of opening and closing valves in xu7/l3 engine. in this study, considering the strength and accuracy of simulation gt-power software in researches on the internal combustion engine...