نتایج جستجو برای: synthetic promoter

تعداد نتایج: 228934  

Journal: :BioTechniques 2008
Stephan Braatsch Søren Helmark Harald Kranz Brian Koebmann Peter Ruhdal Jensen

System-oriented applications of genetic engineering, such as metabolic engineering, often require the serial optimization of enzymatic reaction steps, which can be achieved by transcriptional fine-tuning. However, approaches to changing gene expression are usually limited to deletion and/or strong overexpression and rarely match the desired optimal transcript levels. A solution to this all-or-n...

2009
Jason L. Nathanson Roberto Jappelli Eric D. Scheeff Gerard Manning Kunihiko Obata Sydney Brenner Edward M. Callaway

Short cell-type specific promoter sequences are important for targeted gene therapy and studies of brain circuitry. We report on the ability of short promoter sequences to drive fluorescent protein expression in specific types of mammalian cortical inhibitory neurons using adeno-associated virus (AAV) and lentivirus (LV) vectors. We tested many gene regulatory sequences derived from fugu (Takif...

2012
Fernando Guerrero Verónica Carbonell Matteo Cossu Danilo Correddu Patrik R. Jones

The ethylene-forming enzyme (EFE) from Pseudomonas syringae catalyzes the synthesis of ethylene which can be easily detected in the headspace of closed cultures. A synthetic codon-optimized gene encoding N-terminal His-tagged EFE (EFEh) was expressed in Synechocystis sp. PCC 6803 (Synechocystis) and Escherichia coli (E. coli) under the control of diverse promoters in a self-replicating broad ho...

2014
Sujata Vijay Sohoni Alessandro Fazio Christopher T. Workman Ivan Mijakovic Anna Eliasson Lantz

The objective of this study was the application of the synthetic promoter library (SPL) technology for modulation of actinorhodin production in Streptomyces coelicolor A3(2). The SPL technology was used to optimize the expression of a pathway specific positive transcriptional regulator ActII orf4, which activates the transcription of the S. coelicolor actinorhodin biosynthetic gene cluster. The...

2013
Chunyue Chai Yanling Lin Danyu Shen Yuren Wu Hongjuan Li Daolong Dou

Identification of pathogen-inducible promoters largely lags behind cloning of the genes for disease resistance. Here, we cloned the soybean GmaPPO12 gene and found that it was rapidly and strongly induced by Phytophthorasojae infection. Computational analysis revealed that its promoter contained many known cis-elements, including several defense related transcriptional factor-binding boxes. We ...

Journal: :The Journal of biological chemistry 1985
S A Camper Y A Yao F M Rottman

A 1-kilobase DNA fragment containing the promoter of the bovine prolactin gene was fused to the chloramphenicol acetyltransferase gene and the activity of the promoter was assayed by transfection of the fusion gene into COS-1, HeLa, and GH3 cells. Transcription of the chloramphenicol acetyltransferase gene driven by the prolactin promoter was detected only in GH3 cells, a rat pituitary tumor ce...

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