a new strategy for construction of synthetic gene encoding human basic fibroblast growth factor comprising dna annealing-ligation and augmentation by polymerase chain reaction was introduced. the sequence of the gene and corresponding amino acid chain were modified in order to increase stability of the protein. first, 300 bp and 160 bp fragments of the gene were assembled from 18 oligonucleotid...

A new strategy for construction of synthetic gene encoding human basic fibroblast growth factor comprising DNA annealing-ligation and augmentation by polymerase chain reaction was introduced. The sequence of the gene and corresponding amino acid chain were modified in order to increase stability of the protein. First, 300 bp and 160 bp fragments of the gene were assembled from 18 oligonucleotid...

Synthetic gene networks can be used to control gene expression and cellular phenotypes in a variety of applications. In many instances, however, such networks can behave unreliably due to gene expression noise. Accordingly, there is a need to develop systematic means to tune gene expression noise, so that it can be suppressed in some cases and harnessed in others, e.g. in cellular differentiati...

MOTIVATION Synthetic biology aims to develop the artificial gene networks with desirable behaviors using systematic method. These networks with desired behaviors could be constructed using diverse biological parts, which may limit the development to complex synthetic gene networks. Fortunately, some well-characterized promoter libraries for engineering gene networks are widely available. Thus, ...

One of the goals of synthetic biology is to build regulatory circuits that control cell behavior, for both basic research purposes and biomedical applications. The ability to build transcriptional regulatory devices depends on the availability of programmable, sequence-specific, and effective synthetic transcription factors (TFs). The prokaryotic clustered regularly interspaced short palindromi...

objective: escherichia coli (e.coli) o157:h7 is one of the most important pathogenic causes of hemorrhagic colitis in humans. cattle are the main reservoirs of this bacteria and vaccination is a key mechanism for its control. the intimin, translocated intimin receptor (tir), and espa proteins are virulence factors expressed by the lee locus of enterohemorrhagic e. coli. espa protein is a member...