نتایج جستجو برای: sybr green

تعداد نتایج: 140135  

2008
Wun S. Chao

Real-time polymerase chain reaction (real-time PCR), also known as quantitative PCR, is used to determine relative gene expression or to quantify exact levels of mRNA in cells or tissues. Before the advent of real-time PCR, the major difficulty associated with traditional quantitative or semiquantitative PCR was to ensure that PCR reactions were quantified within the exponential phase of amplif...

2014
Zahra Vali Abbasali Raz Hanieh Bokharaei Mohammad Nabavi Mohammad Hassan Bemanian Mina Sharifi Yazdi Navid Dinparast Djadid

BACKGROUND Asthma is caused by the combination of different factors. Current concepts of asthma pathogenesis emphasize on gene-environment interactions. Mega-genome scanning projects revealed that different Single Nucleotide Polymorphisms (SNPs) are related to asthma susceptibility. rs7216389-T is one of them that is related to childhood asthma and its effect on childhood asthma severity has be...

Journal: :Memorias do Instituto Oswaldo Cruz 2003
Claudia C Rubiano Moises Wasserman

A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10 to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.

Journal: :Applied and environmental microbiology 2003
Dario De Medici Luciana Croci Elisabetta Delibato Simona Di Pasquale Emma Filetici Laura Toti

The objective of this study was to develop a rapid, reproducible, and robust method for detecting Salmonella enterica serotype Enteritidis in poultry samples. First, for the extraction and purification of DNA from the preenrichment culture, four methods (boiling, alkaline lysis, Nucleospin, and Dynabeads DNA Direct System I) were compared. The most effective method was then combined with a real...

2013
Rocio Sanjuan-Jimenez Juan D. Colmenero Pilar Bermúdez Antonio Alonso Pilar Morata

Some sites of extrapulmonary tuberculosis and focal complications of brucellosis are very difficult to differentiate clinically, radiologically, and even histopathologically. Conventional microbiological methods for the diagnosis of extrapulmonary tuberculosis and complicated brucellosis not only lack adequate sensitivity, they are also time consuming, which could lead to an unfavourable progno...

Journal: :Journal of infection in developing countries 2016
Idrissa Diawara Khalid Katfy Khalid Zerouali Houria Belabbes Naima Elmdaghri

INTRODUCTION Acute bacterial meningitis is one of the most severe infectious diseases. Rapid, accurate, and inexpensive diagnosis of bacterial meningitis is crucial for patient management. This study describes a duplex real-time (RT) PCR assay for detection of Neisseria meningitidis and Streptococcus pneumoniae in the cerebrospinal fluid (CSF) for meningitis diagnosis using SYBR Green-based RT-...

Journal: :Clinical chemistry 2006
Mark G Herrmann Jacob D Durtschi L Kathryn Bromley Carl T Wittwer Karl V Voelkerding

BACKGROUND DNA melting analysis for genotyping and mutation scanning of PCR products by use of high-resolution instruments with special "saturation" dyes has recently been reported. The comparative performance of other instruments and dyes has not been evaluated. METHODS A 110-bp fragment of the beta-globin gene including the sickle cell anemia locus (A17T) was amplified by PCR in the presenc...

Journal: :Singapore medical journal 2007
Y K Yong R Thayan H T Chong C T Tan S D Sekaran

INTRODUCTION Dengue fever and dengue haemorrhagic fever currently rank highly among the newly-emerging infectious diseases, and are considered to be the most important arboviral disease worldwide. The definitive diagnosis is culture analysis, but practical considerations limit its use. Also, the period for viral detection is limited. Within a day or two after fever subsides, rising levels of an...

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