Brain trimming through defined neuroanatomical landmarks is recommended to obtain consistent sections in rat toxicity studies. In this article, we describe a matrix-guided trimming protocol that uses channels to reproduce coronal levels of anatomical landmarks. Both setup phase and validation study were performed on Han Wistar male rats (Crl:WI(Han)), 10-week-old, with bodyweight of 298 ± 29 (S...

Previous work showed that, in the presence of DNA-dependent protein kinase (DNA-PK), Artemis slowly trims 3'-phosphoglycolate-terminated blunt ends. To examine the trimming reaction in more detail, long internally labeled DNA substrates were treated with Artemis. In the absence of DNA-PK, Artemis catalyzed extensive 5'-->3' exonucleolytic resection of double-stranded DNA. This resection require...

Glycosidase trimming inhibitors may be used to study contribution of N-linked glycan moieties in T cell function. We have studied the effects of castanospermine (Cas), swainsonine (Swain), 1-deoxynojirimycin (dNM), and 1-deoxymannojirimycin (dMM) on T cell activation and differentiation. Our analysis included a new dNM derivative, N-pentyl-1-deoxynojirimycin (pentyldNM). Previous reports showed...

T he function of the p53 tumor suppressor protein is finely tuned through a myriad of interactions with other proteins. These interactions can lead to posttranslational modifications that regulate p53 stability, DNA binding, or promoterspecific transcriptional activation. A number of p53 binding proteins serve as cofactors that participate in the recruitment of p53 to specific promoters and fac...

In robust nonparametric kernel regression context,weprescribemethod to select trimming parameter and bandwidth. Through solving estimating equations, we control outlier effect through combining weighting and trimming. We show asymptotic consistency, establish bias, variance properties and derive asymptotics. © 2016 Elsevier B.V. All rights reserved.

Before the adoption of an automated system for optimizing edging and trimming in hardwood mills, the performance of present manual systems must be evaluated to provide a basis for comparison. A study was made in which lumber values recovered in actual hardwood operations were compared to the output of a computer-based procedure for edging and trimming optimization. The optimization procedure wa...