Free abscisic acid (ABA) content in suspensors, embryos, and integuments was determined during seed development of Phaseolus coccineus. A highly specific and sensitive solid-phase radioimmunoassay based on a monocional antibody raised against free (S)-ABA was used for ABA quantification. Very small amounts of ABA were detected in the suspensor during initial stages of development; later two pea...

Embryogenic tissues of Abies alba Mill. were cryopreserved using the slow-freezing approach. Four cell lines incubated for 24 h on a medium with 0.5 M sorbitol and pre-treated 5% DMSO. Subsequently, frozen at cooling rate 1 °C min-1 to -40 transferred liquid nitrogen 72 hours. After thawing in water bath 40 °C, cultivated proliferation medium. All tested recovered, but variations regrowth frequ...

In the context of a symbiotic plant-fungus interaction study concerning Cattleya purpurata, we focused on some aspects seed morphology and biology, early stages seedling development. Seed was characterized using light scanning electron microscopy. vitro germination capability evaluated, comparing asymbiotic methods. The seeds overall comparable to that other congeneric species, showing classica...