A third-generation biosensor for superoxide anion (O2-) was developed by immobilizing superoxide dismutase (SOD) on a self-assembled monolayer of cysteine on gold electrode; i.e., a SOD/cysteine-modified gold electrode (SOD/Cys/Au) was fabricated. A rapid and direct electron transfer of SOD was realized at the gold electrode by using the cysteine molecule as an electron-transfer promoter. The p...

The methanol extracts of the fruiting bodies of Ganoderma mastoporum collected in Vietnam was purified to afford eight compounds, including three triterpenoids and five steroids. The purified compounds were examined for their inhibitory effects against superoxide anion generation and elastase release. Among the tested compounds, ergosta-4,6,8(14),22-tetraen-3-one (3) exhibited the most signific...

The effect of pentoxifylline (Trental) on the phagocytic capacity, cAMP levels, and superoxide anion production of human peripheral blood monocytes and polymorphonuclears (PMNs) was studied. The drug inhibited the phagocytosis of latex particles by both monocytes and PMNs in a dose-dependent manner. In addition, superoxide anion production during the phagocytic process was also reduced followin...

Superoxide anion release from polymorphonuclear leukocytes stimulated by concanavalin A was markedly enhanced by the simultaneous addition of cytochalasin A at concentrations of 2x10-s to 4x10-1M. These concentrations of cytochalasin A did not affect the phagocytosis of diisodecyl phthalate emulsion. These concentrations were far lower than those of cytochalasins B, C, D and E that also enhance...

In this study, n-butanol fraction (KBF) and aqueous fraction (KAF) isolated from methanol extract of Koelreuteria paniculata Laxm. leaves were evaluated for their antioxidant potential using (ABTS) 2, 2-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), DPPH (2,2-diphenyl-2-picrylhydrazyl) assay, Reducing power assay and Superoxide anion radical scavenging assay. These fractions were also studi...

Neutrophils are the predominant inflammatory cells found in vaginal discharges of patients infected with Trichomonas vaginalis. In this study, we examined superoxide anion (O2 (.-)) production by neutrophils activated by T. vaginalis. Human neutrophils produced superoxide anions when stimulated with either a lysate of T. vaginalis, its membrane component (MC), or excretory-secretory product (ES...

The aim of the present study was to investigate whether a dietary supplementation of -lipoic acid could prevent blood pressure elevation, insulin resistance, and the increase in aorta superoxide anion production in a new experimental model of hypertension associated with insulin resistance. Sprague-Dawley rats were given 10% D-glucose in their drinking water combined either with a normal chow d...

The involvement of mitochondrial electron transport chain (METC)-derived superoxide anion radical in cell protooncogene activation, mitogenic responses, and cancerous growth has recently received much attention. In order for METC-derived superoxide to participate in any of the above processes, its exit from mitochondria would be a critical step. Detection of intracellular superoxide showed that...

Left ventricular hypertrophy (LVH) is an independent marker of mortality in hypertension. Although the mechanisms contributing to LVH are complex, inflammation and oxidative stress may favor its development. We analyzed the association of the phagocytic NADPH oxidase-mediated superoxide anion release and LVH in patients with essential hypertension and the role of cardiotrophin-1 (CT-1) and inte...

Purpose: To investigate the photoprotective effect of (-)-epigallocatechin gallate (EGCG), one of tea catechins, on human skin fibroblast (HSF) irradiated by ultraviolet A. Methods: HSF cells were incubated in serum-free Dulbecco's Modified Eagle's Medium (DMEM) with or without EGCG for 2 h, and then irradiated by UV A. Blank (control) was incubated in DMEM without EGCG and UV A-irradiation. Ce...