In a previous report (1) we have shown that a small fraction of the sulfur of injected thiocyanate is converted to sulfate by the rat. The conversion of thiocyanate to sulfate had been predicted to occur as the result of the physiological activity of thiocyanate ion which is known to be a goitrogen and a hypotensive agent. Our results could not be taken as unequivocal evidence for the metabolis...

Using 1-(2-nitrophenyl)ethyl sulfate (caged sulfate) as a photoactivatable caged proton, we could induce complete acid unfolding of myoglobin with a single nanosecond laser pulse. This was possible because of the high ( approximately mM) concentration of protons released by the photolabile compound. The ability of the compound to produce a large pH jump arises because the other photoproducts (2...

Thermochemical sulfate reduction experiments with simple amino acid and dilute concentrations of sulfate reveal significant degrees of mass-independent sulfur isotope fractionation. Enrichments of up to 13‰ for (33)S are attributed to a magnetic isotope effect (MIE) associated with the formation of thiol-disulfide, ion-radical pairs. Observed (36)S depletions in products are explained here by c...

A simple method for concentrating, isolating, and purifying PF/cholera enterotoxin from culture supernatant fluids is presented. Precipitation with dextran sulfate and ammonium sulfate, followed by gel filtration and ion-exchange chromatography, yielded PF/enterotoxin of high biological potency. Although not completely free from traces of vibriocidal antibody-stimulating activity, the resultant...