Large granule fractions, containing about 80% of the cytochrome oxidase of the tissue, were isolated from rat liver and used to prepare thin pellicles of packed particles which were submitted to quantitative electron microscopic examination. Various parameters describing the mitochondrial population were determined by measuring the size and number of mitochondrial profiles in sections, and the ...

We report a quantitative subcellular localization study of calmodulin and the subcellular distribution of calmodulin-binding proteins in normal and virus-transformed chicken embryo fibroblasts. We developed homogenization conditions for fibroblasts which give maximal cell disruption with minimal organelle destruction, prepared and characterized subcellular fractions, and measured the levels and...

The distribution of rat liver epoxide hydrolase in various subcellular fractions was investigated by immuno-electron-microscopy. Ferritin-linked monospecific anti-(epoxide hydrolase) immunoglobulins bound specifically to the cytoplasmic surfaces of total microsomal preparations and smooth and rough microsomal fractions as well as the nuclear envelope. Specific binding was not observed when the ...

The levels of pyridine nucleotides in tissues have been the subject of a large number of reports. Much interest has been focused on the ratios of oxidized to reduced coenzymes in different pathological conditions (1). Recently, Glock and McLean (2) have reported the important observation that reduced TPN is present in whole rat liver in comparatively large amounts, whereas the concentration of ...

Rat liver microsomes and microsomal subfractions isolated by density equilibration were submitted to a quantitative morphological and biochemical analysis. The total area of the endoplasmic reticulum was estimated at 7.3 m(2) per g of liver. The microsome fraction contained 2.8 mg of phospholipids and 6.7 mg of proteins per m(2) of membrane area. After correction for ribosomal and intracisterna...

Anglerfish islets were homogenized in 0.25 M sucrose and separated into seven separate subcellular fractions by differential and discontinuous density gradient centrifugation. The objective was to isolate microsomes and secretory granules in a highly purified state. The fractions were characterized by electron microscopy and chemical analyses. Each fraction was assayed for its content of protei...

Some characteristic enzymatic activities were determined in chick embryonic cartilage and compared with the analogous activities in bone and liver. Chondrocytes were isolated, broken by sonication, and subjected to subcellular fractionation to yield a nuclear pellet, the mitochondrial, lysosomal, and microsomal fractions, and the high speed supernatant solution . It was established that these f...

1. Rat cerebral-cortex slices were incubated with 32pl, acetylcholine and eserine for periods of 10min and 2h. The specific radioactivity of phosphatidylinositol was elevated during these treatments by 36 and 106% respectively. 2. The specific radioactivities of the phosphatidylinositol in different cell structures were determined after subcellular fractionation. They were highest in the nuclea...