Analysis of protein structures typically beginswith decomposition of the structure intomore basic units called structural domains. The underlying goal is to reduce a complex protein structure to a set of simpler, yet structurallymeaningful units, each ofwhich can be analyzed independently. Structural semi-independence of domains is their hallmark: domains often have compact structure that can f...

A number of componenets of cytoskeleton are very large proteins which are in their complete native form non amenable to the structure determination via X-ray diffraction techniques due to their non-globular shape and intrinsic flexibility which hinder the formation of suitable crystals. Dissecting such proteins into their representative building blocks-domains and subsequent structural studies ...

Purified rabies virions, unlabeled or labeled with radioactive amino acids or d-glucosamine, were dissociated into their polypeptides by treatment with sodium dodecyl sulfate in a reducing environment and fractionated by electroiphoresis in sodium dodecyl sulfate-containing polyacrylamide gel. The molecular weights of individual polypeptides were estimated by comparison of their rate of migrati...

A moment analysis of the self-intermediate scattering function is used to reconstruct the distribution of structural displacements in the small protein myoglobin as a function of time, temperature and environment. Model-independent information is obtained on mean squared displacements and the Gauss-deviation. Two types of molecular motions were identified: rotational jumps of side chains and sm...

Viral Gastroenteritis Unit, Division of Viral Diseases, Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia 303331*; Laboratory of Virus Morphology, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, China2; Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 7703O3; Department of Pediatrics, The Johns Hopkins Hospit...

In biological macromolecules, structural patterns (motifs) are often repeated across different molecules. Detection of these common motifs in a new molecule can provide useful clues to the functional properties of such a molecule. We formulate the problem of identifying a given structural motif (pattern) in a target protein (example) and discuss the notion of complete matches vis-a-vis partial ...

Polyacrylamide gel analysis of the structural proteins of African and Asian strains of Chikungunya virus, an alphavirus, showed that both strains contain three structural proteins: glycosylated E1 and E2, embedded in the viral envelope, and a nonglycosylated nucleocapsid protein. In pulse-chase experiments the precursor protein PE2 was chased into glycoprotein E2, which migrated slightly faster...

Introduction: Vaccine studies against Pseudomonas aeruginosa have often focused on outer membrane proteins (OPRs) due to their potent stimulation of the immune response. Using major outer membrane proteins of cell walls (mOMPs) of P. aeruginosa and other Gram-negative bacteria actively stimulate the immune system without any toxic side effects. Moreover, these antigens show immunological cross-...