background: among the most important parasitic disease, causing diarrhea, gi­ardia lamblia is noteworthy. nowadays detection methods for these parasites in­clude parasitological methods such as microscopic examination. the sensitivity of these methods relies on the expertise and experience of examiners. in contrast, molecular methods such as pcr are less dependent on the expertise of the exam­i...

BACKGROUND Stool microscopy, the commonly used test for diagnosis of intestinal amebiasis, is unreliable as it does not differentiate Entamoeba histolytica, the causative agent for amebiasis, from non-pathogenic Entamoeba dispar and Entamoeba moshkovskii. METHODS Two hundred and forty-six stool samples collected between January to February 2009, were examined microscopically for E. histolytic...

BACKGROUND Strongyloidiasis is mostly an asymptomatic infection and diagnosis of latent infections is difficult due to limitations of current parasitological and serological methods. This study was conducted to set up a PCR-based method for molecular diagnosis of Strongyloides stercoralis infection by detection of copro-DNA in stool samples. METHODS A total of 782 fresh stool samples were col...

The aim of current studies was to validate the direct plating of a stool sample for Listeria monocytogenes detection, using selective medium Palcam agar with Palcam selective supplement. Validation was performed using stool samples collected from healthy humans inoculated with Listeria sp. strains. Stool samples were frozen to determine the influence of freezing on method robustness. The presen...

BACKGROUND Strongyloides stercoralis, the chief causative agent of human strongyloidiasis, is a nematode globally distributed but mainly endemic in tropical and subtropical regions. Chronic infection is often clinically asymptomatic but it can result in severe hyperinfection syndrome or disseminated strongyloidiasis in immunocompromised patients. There is a great diversity of techniques used in...

background: chronic diarrhea is a common problem in patients with symptoms related to gastro-intestinal tract. some of these patients present with chronic non-bloody, watery diarrhea and no specific clinical, laboratory, or endoscopic findings. the aim of the present study was to investigate the value of histological findings in patients with chronic diarrhea who have normal or non-specific end...