Colchicine was used as chemical mutagen for In vitro induction of polyploids. Nodal segments of In vitro grown marigold plantlets were cultured on MS medium supplemented with four different concentrations of colchcine (0.0%, 0.001%, 0.01% and 0.05%) for 12h, separately. After treatment, nodal segment were shifted to colchicine free MS medium for normal growth. Highest mortality rate (72.74%) an...

Stomata are pores in the plant epidermis that control carbon dioxide uptake and water loss. They are major regulators of global carbon and water cycles [1]. Several signaling components that regulate stomatal development have been characterized. These include a putative secretory peptide EPF1, LRR receptor components TMM and ER, and a peptidase SDD1 [2-4]. We have identified EPF2, a peptide rel...

Stomatal pores are microscopic structures on the epidermis of leaves formed by 2 specialized guard cells that control the exchange of water vapor and CO(2) between plants and the atmosphere. Stomatal size (S) and density (D) determine maximum leaf diffusive (stomatal) conductance of CO(2) (g(c(max))) to sites of assimilation. Although large variations in D observed in the fossil record have bee...

In most plants, stomata are located only on the abaxial leaf surface (hypostomy), but many plants have stomata on both surfaces (amphistomy). High light and herbaceous growth form have been hypothesized to favor amphistomy, but these hypotheses have not been rigorously tested together using phylogenetic comparative methods. I leveraged a large dataset including stomatal ratio, Ellenberg light i...

Wild-type stomata are distributed nonrandomly, and their density is controlled by endogenous and exogenous factors. In the Arabidopsis mutant stomatal density and distribution1-1 (sdd1-1), the establishment of the stomatal pattern is disrupted, resulting in stomata clustering and twofold to fourfold increases in stomatal density. The SDD1 gene that encodes a subtilisin-like Ser protease is expr...

net photosynthesis rate (pn), stomatal conductance (gs) and transpiration rate (e) of anise hyssop were measured during the four cloudless days, in reference to diurnal fluctuations of leaf temperature (tleaf), leaf vapor pressure deficit (vpd leaf) and photosynthetic photon flux density (ppfd) in well watered (ww), stressed (s) and recovered (r) plants. an analysis of measured data showed that...

A goal of modern agriculture is to improve plant drought tolerance and production per amount of water used, referred to as water use efficiency (WUE). Although stomatal density has been linked to WUE, the causal molecular mechanisms have yet to be determined. Arabidopsis thaliana GT-2 LIKE 1 (GTL1) loss-of-function mutations result in increased water deficit tolerance and higher integrated WUE ...