Steviol glycosides (SG's) from Stevia rebaudiana (Bertoni) have been used as a natural low-calorie sweeteners. Its aftertaste bitterness restricts its use for human consumption and limits its application in food and pharmaceutical products. In present study, we have performed computational analysis in order to investigate the interaction of two major constituents of SG's against homology model ...

Stevia rebaudiana Bertoni and Mentha piperita are plants that generate interest mainly due to the presence of bioactive compounds in their leaves, such as phenolics. Studies indicate phenolics have pharmacological therapeutic properties, including antioxidant activity. Phenolic may be affected by type fertilization. For this reason, organic chemical fertilization were evaluated along with Resul...

Background: Stevia rebaudiana Bertoni, an important anti-diabetic medicinal plant, becomes an inevitable alternative to sugar. Due to the propagation difficulties, tissue culture is the best alternative for rapid mass propagation of stevia plants. Objective: The present study was conducted to optimize a protocol for rapid micropropagation of Stevia rebaudiana by shoot tip explants and to inv...

The genomic DNA polymorphism and phytochemical variation of Stevia rebaudiana Bertoni was investigated by RAPD-PCR and HPLC analysis. For initial PCR screening, about hundred oligo-nucleotide primers were used to amplify the genomic DNA from three accessions of S. rebaudiana (L1 to L3). The concentration of stevioside content was quantified by using HPLC analysis. A total of 9 primers were fina...

Two additional novel minor diterpene glycosides were isolated from the commercial extract of the leaves of Stevia rebaudiana Bertoni. The structures of the new compounds were identified as 13-{β-D-glucopyranosyl-(1 → 2)-O-[β-D-glucopyranosyl-(1 → 3)-β-D-glucopyranosyl-oxy} ent-kaur-16-en-19-oic acid {β-D-xylopyranosyl-(1 → 2)-O-[β-D-glucopyranosyl-(1 → 3)]-O-β-D-glucupyranosyl-ester} (1), and 1...

Degradation of rebaudioside M, a minor sweet component of Stevia rebaudiana Bertoni, under conditions that simulated extreme pH and temperature conditions has been studied. Thus, rebaudioside M was treated with 0.1 M phosphoric acid solution (pH 2.0) and 80 °C temperature for 24 h. Experimental results indicated that rebaudioside M under low pH and higher temperature yielded three minor degrada...

Stevia rebaudiana is a sweet herb of the Astraceae family, which is cultivated for the natural sweeteners it contains. The aim of this study was to assess the effect of drought, simulated by the application of polyethylene glycol (5%, 10%, and 15% w/v), on the content of steviol glycosides (SVglys) and transcription levels of six genes involved in the biosynthesis of these natural sweeteners. T...

Anti-inflammatory activities of methanolic extract of callus and intact plant part of Stevia rebaudiana(Bert.) Bertoni was studied for the first time. Acute inflammation model, carrageenan induced rat hind paw edema model was employed to investigate the anti-inflammatory activity. The methanolic extract from fresh callus cultures of Stevia rebaudianacultured on Murashige and Skoog (MS) medium f...