4. Biochemistry of palm oil ..................................................................................................................................... 510 4.1. Fruit development and oil deposition ...................................................................................................... 510 4.2. Changes in lipid class and fatty acid composition during development of the o...

MEMBRANE TOPOLOGY OF MOUSE STEAROYL-COA DESATURASE 1 Weng Chi Man*, Makoto Miyazaki*, Kiki Chu*, and James M. Ntambi* From the Departments of *Biochemistry and ¶Nutritional Sciences, University of Wisconsin-Madison, Madison, WI 53706 Running Title: Mouse SCD1 Topology Address correspondence to: James M. Ntambi, Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madi...

Here we present an innovative computational-based drug discovery strategy, coupled with machine-based learning and functional assessment, for the rational design of novel small molecule inhibitors of the lipogenic enzyme stearoyl-CoA desaturase 1 (SCD1). Our methods resulted in the discovery of several unique molecules, of which our lead compound SSI-4 demonstrates potent anti-tumor activity, w...

The biosynthesis pathway to diadinoxanthin and fucoxanthin was elucidated in Phaeodactylum tricornutum by a combined approach involving metabolite analysis identification of gene function. For the initial steps leading to β-carotene, putative genes were selected from the genomic database and the function of several of them identified by genetic pathway complementation in Escherichia coli. They ...

Recent studies have shown that a tumor-supportive microenvironment is characterized by high levels of pro-inflammatory and pro-angiogenic eicosanoids derived from omega-6 (n-6) arachidonic acid (AA). Although the metabolic pathways (COX, LOX, and P450) that generate these n-6 AA eicosanoids have been targeted, the role of endogenous AA production in tumorigenesis remains unexplored. Delta-6 des...

The cultivated peanut (Arachis hypogaea L.) is an allotetraploid composed of A and B genomes. The phylogenetic relationship among the cultivated peanut, wild diploid, and tetraploid species in the section Arachis was studied based on sequence comparison of stearoyl-ACP desaturase and oleoyl-PC desaturase. The topology of the trees for both fatty acid desaturases displayed two clusters; one clus...

BACKGROUND/AIMS Muscle bioactive lipids accumulation leads to several disorder states. The most common are insulin resistance (IR) and type 2 diabetes. There is an ongoing debate which of the lipid species plays the major role in induction of muscle IR. Our aim was to elucidate the role of particular lipid group in induction of muscle IR. METHODS The analyses were performed on muscle from the...

Developing soybean (Glycine max) seeds respond to a change in growth temperature by changing the level of stearoyl acyl carrier protein desaturase activity in the tissue. After 20 hours in liquid culture, seeds grown at 20 degrees C show an increase in activity while seeds grown at 35 degrees C show a decrease in activity, relative to their preculture levels. Analysis of the enzyme from both gr...