4H-SiC Metal-Oxide-Semiconductor Field Effect Transistors (MOSFETs) with embedded Schottky barrier diodes are widely known to improve switching energy loss by reducing reverse recovery characteristics. However, it weakens the static characteristics such as specific on-resistance and breakdown voltage. To solve this problem, in paper, an Asymmetric Split Gate MOSFET diode (ASG-MOSFET) is propose...

We have tested and compared the performance of 12 different assays representing four different real-time polymerase chain reaction (PCR) chemistries in the context of genetically modified organism detection. Several different molecular beacon, SYBR Green, TaqMan, and MGB assays were designed for the event specific detection and quantification of the 3' integration junction of GTS 40-3-2 (Roundu...

Background: Taqman one-step real-time PCR (RT-PCR) has special importance due to its high sensitivity and specificity in the diagnosis of infectious diseases such as viral infections. In recent pandemic SARS-CoV-2, diagnostic kits based on this method are commonly used for molecular detection. One main systematic errors that misinterpret results is using inaccurate internal control RT-PCR kits....

In this paper, a novel super-junction trench silicon-on-insulator laterally-diffused metal-oxide-semiconductor (SJT SOI LDMOS) power device with additional hole leakage paths to improve single-event burnout (SEB) performance under high liner energy transfer (LET) is proposed for the first time. The electrical characteristics and SEB of SJT LDMOS are both enhanced effectively. replacement lightl...

As micromixers offer the cheap and simple mixing of fluids suspensions, they have become a key device in microfluidics. Their performance can be significantly increased by periodically varying inlet pressure, which leads to non-static flow improved process. In this work, micromixer with T-junction meandering channel is considered. A periodic pulse function for pressure numerically optimized reg...

BACKGROUND The intestinal tract plays an important role in food allergy and the intestinal mucosa barrier is critical for maintenance of its function. The underlying mechanisms of how food allergens modulate the intestinal permeability in inducing intestinal food allergy remain elusive. OBJECTIVE The aim of this study was to explore the mechanism of how food allergens influence the function o...

Deep sequencing of transcriptome (RNA-seq) provides unprecedented opportunity to interrogate plausible mRNA splicing patterns by mapping RNA-seq reads to exon junctions (thereafter junction reads). In most previous studies, exon junctions were detected by using the quantitative information of junction reads. The quantitative criterion (e.g. minimum of two junction reads), although is straightfo...

Differential expression of members of the connexin (Cx) gap junction multigene family permits formation of gap junctions with the varied physiological properties required by different tissues. The aim of this study was to characterize connexin expression and the influence of all-trans-retinoic acid (RA) in mouse gingival epithelial cells (GE1). The cells were treated with RA, and expression of ...

Abstract Additive manufacturing for microfluidics shows potential to boost research and development in biology molecular diagnostics. This paper reports on novel process material optimisation techniques the creation of a monolithic microfluidic chip geometry polymerase chain reaction (PCR) thermocycling using stereolithography (SLA). A two-stage printing protocol with projection SLA is assessed...

BACKGROUND The gap junction plays an important role in spreading of apoptotic and necrotic signals from injured and stressed cells to the neighboring viable cells. The present study was performed to investigate the important role of gap junction communication on rabbits' explosive brain injury. METHODS Explosion of paper detonators was used to create explosive brain injury model in 60 rabbits...