The copper protein plastocyanin has been found to be an essential component of the photosynthetic electron transport chain of Chlamydomonas reinhardi, and in this paper we describe a method for its isolation and purification from the wild-type strain. In addition, we describe some of its properties and compare them with those reported for spinach plastocyanin.The plastocyanin was extracted from...

The role of hexokinase in carbohydrate degradation in isolated, intact chloroplasts was evaluated. This was accomplished by monitoring the evolution of 14CO2 from darkened spinach (Spinacia oleracea), maize (Zea mays) mesophyll, and Chlamydomonas reinhardtii chloroplasts externally supplied with 14C-labeled fructose, glucose, mannose, galactose, maltose, and ribose. Glucose and ribose were the ...

ABSTRACT A curtovirus associated with a disease of spinach was isolated in southwest Texas during 1996. Disease symptoms included severe stunting and chlorosis, with younger leaves curled, distorted, and dwarfed. Viral DNA was purified and an infectious clone obtained. Agroinoculation using a construct bearing full-length tandem repeats of the cloned viral genome resulted in systemic infection ...

Aims: To evaluate the nematicidal potential of wild spinach powder in combination with fresh chopped leaves of different plants viz., Indian mallow, Mexican poppy, Ivy gourd, Trailing eclipta, Wild eggplant and Black pigweed against Meloidogyne incognita on plant growth characters of tomato cv. K-21. Place and Duration of Study: A glasshouse pot experiment was carried out in the Department of B...

In order to investigate the effects of allelo-pathic substances Conyza canadensis on seed germination and seedlings, this study was designed explore allelopathic water extract (CCE) pakchoi, spinach lettuce. Germination rates three crops (pakcholi, spinach, lettuce) during germination, root length, seedling height, fresh weight two (pakcholi were measured under different concentrations CCE trea...

Fusarium wilt of spinach, caused by oxysporum f. sp. spinaciae, is an important disease during warm conditions in production regions with acid soils, yet little known about what confers pathogenicity to spinach F. spinaciae genetically. To identify candidate fungal genes that contribute wilt, each 69 geographically diverse isolates was tested for on three inbreds. Thirty-nine identified as quan...

Green spinach is a vegetable that can be consumed by its leaves as green and used processed food, both main menu snack. Spinach has many nutritional contents such protein, minerals, calcium, iron vitamins needed in the body. Iron contained supplement, if large doses long time cause damage to intestinal lining, abnormalities pH of body, shock liver failure. An alternative meet needs done consumi...

Spinach leaves, containing several active components, including flavonoids, exhibit antioxidative, antiproliferative, and antiinflammatory properties in biological systems. Spinach extracts have been demonstrated to exert numerous beneficial effects, such as chemo- and central nervous system protection and anticancer and antiaging functions. In this review article, we present a compilation of d...

Acylation Mechanism, Galactolipid Synthesis The occurrence of acylgalactosylglycerol (AGG) in spinach chloroplasts is proposed. In lipid se­ parations of whole spinach leaves this fraction is hidden by variable amounts of extraplastidary steryl glucoside (SG) which depended on the physiological state of the leaf material. Beside the phospholipid fraction, this lipid mixture with unique chromato...

Starch debranching enzyme was purified from intact spinach (Spinacia oleracea L. cv Vital) chloroplasts and from a spinach leaf extract using affinity chromatography on Sepharose 6B-bound cycloheptaamylose (Schardinger beta-dextrin). The enzyme from both sources was homogeneous upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Spinach leaf debranching enzyme appears to consist of ...