Successful spermatogonial transplantation requires depletion of the host germ cells to allow efficient colonization of the donor spermatogonial stem cells. Although a sterilizing drug, busulfan (Myleran), is commonly used for preparing a recipient mouse before transplantation, the optimal dose of this drug has not yet been defined. The present study investigated the effects of different doses o...

background: the basis of spermatogenesis is the spermatogonial stem cells (sscs). the concentration of sscs is very small. however, a system that supports the proliferation and maintenance of sscs in vitro could be used to preserve and expand sscs numbers as well as increase success in transplantation. it is a new avenue to restore spermatogenesis in azoospermia subjects. objective: proliferati...

Introduction: One of the vital enzymes during spermatogenesis, which is one of the pluripotency factors of stem cells and contributes to maintaining their pluripotency is alkaline phosphatase. ZBTB16 and ZFP proteins are critical elements in stem cells which are expressed in pluripotent stem cells and maintain their pluripotency due to their role in messaging pathways. Material & Methods: The ...

Regulation of the germ stem cell niche as the foundation for adult spermatogenesis: A role for miRNAs?' Seminars in Cell and Developmental Biology, vol 29C, pp. 76-83. General rights Copyright for the publications made accessible via the Edinburgh Research Explorer is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recog...

Stem cells are unique that have the ability to differentiate into with specific functions and renew themselves produce new stem cells. One type of actively studied is Spermatogonial Cells (SSC). SSC a unipotent cell develops spermatozoa in mammalian testis. can be transplanted male testis recipients improve genetic quality livestock propagate valuable livestock. transplantation procedure starte...

The origin and development of the spermatogenic cell lineage is reviewed, as well as spermatogonial kinetics in adult nonprimate mammals in relation to the cycle of the seminiferous epithelium, the emphasis being on spermatogonial stem cells. A hypothesis is presented for the transition from foetal germ cells, gonocytes, to adult type spermatogonia at the start of spermatogenesis. An overview i...

This review looks into the phylogeny of spermatogonial stem cells and describes their basic biological features. We are focusing on species-specific differences of spermatogonial stem cell physiology. We propose revised models for the clonal expansion of spermatogonia and for the potential existence of true stem cells and progenitors in primates but not in rodents. We create a new model for the...