The effect of cold storage of testes and epididymides at 4 °C for 12 h on the cryopreservation capacity of epididymal feline sperm was evaluated. Ten domestic cats were castrated, and testes and epididymides collected. Specimens were randomly assigned to two groups: in Group A, epididymal samples were immediately processed and frozen in 0.25-ml straws; in Group B, both testes and epididymides w...

BACKGROUND When male animals die, spermatozoa within the body of animal will be degenerated. Because of unique chromatin structure of sperm, maybe this degeneration is different from other cells. However there is not any research which considered directly the integrity of sperm DNA by keeping the cadaver in refrigerator. OBJECTIVE The aim of this study was to assess viability, total motility ...

Sperm quantity and quality during storage may be constraints acting on female fecundity and hence fitness. In Hymenoptera, the importance of sperm quality has rarely been considered, despite its central role in reproductive strategies and especially in sex ratio control. In these insects, fertilized eggs develop into females and unfertilized eggs into males. Experiments were conducted on the fe...

background: control of the medium osmolarity and temperature during long or short time sperm manipulation is essential. objective: the objectives of the present study were to find the effects of different osmolarities of modified tyrode's solution and milk on the bull sperm during incubation at above zero temperatures. materials and methods: semen samples were collected twice from five najdi bu...

background: sperm vitrification is a technique of ice and cryoprotectant free cryopreservation by direct plunging of sperm suspension into liquid nitrogen (ln2). the aim of this study was to investigate the influence of cryoprotectant free-vitrification on human sperm fine structure by msome technology and the fertility potential by zona binding assay (zba). methods: 20 normo-ejaculates were pr...

BACKGROUND The non-lethal collection of sperm from live males is an important component for multiple captive-breeding techniques, including assisted reproductive technology (ART) protocols, sperm cryopreservation and in vitro fertilization. However, in amphibians, the type and amount of hormone necessary to induce spermiation can be highly variable, even among closely related species. We are un...

Cooling method was proposed to maintain the sperm quality for several days. Nevertheless, during this procedure, sperm is encountered to “cold shock”, and its quality decreases time-dependently. This study was designed to improve the in vitro sperm preservation methods. Thirty normal semen samples were examined in Shiraz, Iran, 2017. Fifteen samples were incubated at 22-27 °C and 15 samples wer...

Background: Ram sperm have a higher polyunsaturated fatty acids and a lower cholesterol/phospholipid ratio than other species which renders the sperm much more vulnerable to oxidative damage caused by reactive oxygen species (ROS). Oxidative stress can affects the integrity of cells at both the structural and sub structural levels. Therefore, a suitable antioxidant may prevent cryo-injuries and...