OBJECTIVE To compare the sperm chromatin dispersion (SCD) test and the terminal uridine nick-end labeling (TUNEL) assay for assessment of sperm DNA damage. DESIGN Prospective comparative experimental study. SETTING Andrology laboratory. PATIENT(S) Twenty subfertile men with unexplained infertility. INTERVENTION(S) Sperm DNA damage was determined in the same semen samples using the TUNEL...

OBJECTIVE This study aimed to assess and compare sperm motility, concentration, and morphology recovery rates, before and after processing through sperm washing followed by swim-up or discontinuous density gradient centrifugation in normospermic individuals. METHODS Fifty-eight semen samples were used in double intrauterine insemination procedures; 17 samples (group 1) were prepared with sper...

Sperm morphology evolves rapidly, resulting in an exceptional diversity of sperm size and shape across animal phyla. This swift evolution has been thought to prevent fertilizations between closely related species. Alternatively, recent correlative analyses suggest that competition among sperm from more than one male may cause sperm diversity, but these hypotheses have not been tested. Here, we ...

introduction: the aim of this study was to evaluate the effect of human sperm mtt viability assay on outcome of intracytoplasmic sperm injection. mtt is a tetrazolium salt, routinely used for cell proliferation and cytotoxicity assays. materials and methods: 50μl of processed semen was treated with mtt solution, while the remaining used as the control. meanwhile, 109 donated human oocytes (meta...

BACKGROUND No reports have been published about age-related sperm malformations in varicocele patients. AIM To investigate the distribution of abnormal sperm characteristics in adolescents, adults and older men with varicocele. SETTING AND DESIGN Records of semen analysis of 143 men aged 14 to 53 years who had evident left-sided varicocele detected by physical examination and confirmed by d...

Introduction: The image quality of computed tomography (CT) can be seriously lowered by metal implants of patients. These implants are known to exert a significant impact on diagnostic accuracy due to artifacts. The current study aimed to assess the usefulness of Metal Artifact Reduction (MAR) software in the reduction of metal artifacts, in comparison to iterative rec...

BACKGROUND The majority of men find the production of a semen sample an embarrassing and stressful experience. Consequently, the availability of an over-the-counter home sperm test, which would reliably and accurately allow the patient to obtain an assessment of fertility potential at their convenience, would be a major benefit. Our objective was to develop and evaluate a home sperm test that p...

The process of fertilization can’t be attributed only to the number of live, motile and normal sperm deposited within the female but especially to its functionality [1]. The hypoosmotic test or hypoosmotic test (HOST) is a functional test, by which the live sperm are subjected to an incubation in a hypoosmotic solution, the sperm with the functional membrane will allow the entry of water by osm...

background: sperm vitrification is a technique of ice and cryoprotectant free cryopreservation by direct plunging of sperm suspension into liquid nitrogen (ln2). the aim of this study was to investigate the influence of cryoprotectant free-vitrification on human sperm fine structure by msome technology and the fertility potential by zona binding assay (zba). methods: 20 normo-ejaculates were pr...