During spermiogenesis, round spermatids are converted into motile sperm in mammals. The mechanisms responsible for sperm morphogenesis are poorly understood. We have characterized a novel protein, RIM-BP3, with a specialized function in spermatid development in mice. The RIM-BP3 protein is associated with the manchette, a transient microtubular structure believed to be important for morphogenes...

Recognition between mammalian gametes occurs when the plasma membrane of the sperm head binds to the zona pellucida (ZP), an extracellular coat surrounding eggs. ZP3, one of three glycoproteins in the ZP, is the egg protein recognized by sperm. A mouse sperm surface protein, sp56 (M(r) = 56,000), has been identified on the basis of its specific affinity for ZP3 (Bleil, J. D., and P. M. Wassarma...

Background: Many sperm characteristics including, motility and morphology are important for normal spermatozoa- oocyte intraction. These features may be altered during vitrification. Motile sperm organelle morphology examination (MSOME) is an unstained real time high magnification technique that analysis viable sperm morphology instantly. This study aimed to investigate the influence of vitrifi...

Flagellar Propulsion The image representing article 2200210, Magdanz, Khalil and co-workers show a collinear bundle under the scanning electron microscope. consists of two sperm cells with opposite orientations. In presence adhesive molecules on heads, assemble into by head-to-head attachment. This unique configuration bundles allows us to understand swimming behavior when against time-periodic...

There is genetic evidence that the two species of Brazilian gray Mazama, Mazama gouazoubira and Mazama nemorivaga, belong to different genera. This study identified significant differences that separated them into distinct groups, based on characteristics of the spermatozoa and ejaculate of both species. The characteristics that most clearly differentiated between the species were ejaculate col...

OBJECTIVE To identify whether sperm characteristics after washing and/or ovulation induction cycle characteristics can predict the occurrence of multiple conception in patients undergoing ovarian stimulation and IUI. DESIGN Retrospective study. SETTING A gynecology clinic and an andrology laboratory at a tertiary care facility. PATIENT(S) One hundred patients with single pregnancies and 2...

Hypercholesterolemia represents a high risk factor for frequent diseases and it has also been associated with poor semen quality that may lead to male infertility. The aim of this study was to analyze semen and sperm function in diet-induced hypercholesterolemic rabbits. Twelve adult White New Zealand male rabbits were fed ad libitum a control diet or a diet supplemented with 0.05% cholesterol....

It was reported that recombinase-A protein (RecA)-coated exogenous DNA was more likely to be integrated into mouse, goat and pig genomes. The objective of this study was to investigate whether integration of exogenous DNA into the rat genome is improved by the recombinase-mediated DNA transfer. Pronuclear microinjection of RecA-coated EGFP or OAMB DNA resulted in a production efficiency of tran...

The dimensions of spermatozoa from different levels of the reproductive tract were measured in 6 buffalo-bulls castrated under local anesthesia ; the same measurements were taken in the semen of 6 breeding buffalo-bulls. During transit from caput to cauda epididymis the sperm showed a significant increase in the length of head and main piece. However, a significant decrease was observed in midd...

In order to extend the static information of immunolabelling sulphogalactolipids in fixed boar spermatozoa, a fluorescent sulphogalactolipid analogue, galactose(3-sulphate)-beta 1-1'[(N-lissamine rhodaminyl)-12-aminodode-canoyl]-sphingosine, was incorporated into plasma membranes of living spermatozoa and its lateral distribution over the sperm head was studied. The fluorescent lipid was enrich...