A simple and sensitive UV spectrophotometric method was developed and validated for the determination of pregabalin in bulk, pharmaceutical formulations and in human urine samples. The method was linear in the range of 0.5-5.0 μg/ml. There is no generally accepted method for the determination of pregabalin. The absorbance was measured at 210 nm. The method was validated with respect to accuracy...

A flow injection method for the quantitative analysis of vancomycin hydrochloride, C66H75Cl2N9O24.HCl (HVCM), based on the reaction with copper (II) ions, is presented. HVCM forms a lilac-blue complex with copper ions at pH≅4.5 in aqueous solutions, with maximum absorption at 555 nm. The detection limit was estimated to be about 8.5×10-5 mol L-1; the quantitation limit is about 2.5×10-4 mol L-1...

Terbinafine Hydrochloride (TH) is a new potent antifungal agent. UV-spectrophotometric method has been developed for the determination of TH in bulk and in tablet dosage form. For the determination of Terbinafine Hydrochloride, solvent system employed was methanol and wavelength of detection was 223 nm. The aim of this study was to develop simple, sensitive, cost effective, accurate, precise, r...

Non-enzymatic posttranslational modifications of bovine serum albumin (BSA) by oxo-compounds, particularly glucose, ribose, glyoxal and glutardialdehyde, have been investigated using a set of modern chromatographic and electrophoretic separation methods. High-performance liquid chromatography (HPLC) alternatively with UV spectrophotometric (diode array) or mass spectrometric (MS) detection, pol...

Simple, precise and economic batch flow injection analysis (FIA)-spectrophotometric methods have been established for simultaneous determination of salbutamol sulfate (SLB) in bulk powder pharmaceutical forms. Both based on diazotization coupling reaction SLB with another drug compound (sulfadimidine) as a safe green agent alkaline medium. At 444 nm, the maximum absorption orange azo-dye produc...

Spectrophotometric methods were developed according to Quality by Design (QbD) approach as per ICH Q8(R2) guidelines for estimation of dolutegravir. QbD was carried out varying various parameters, and these variable parameters designed into Ishikawa diagram. The present work deals with the development sensitive, simple, accurate, precise cost-effective UV-spectrophotometric method determination...

Terbinafine Hydrochloride (TH) is a new potent antifungal agent. Many spectroscopic methods are developed using organic solvents, but inorganic solvents like hydrochloric acid, sodium hydroxide can also be used for the estimation of APIs depending on their solubility. UV-spectrophotometric method has been developed for the determination of TH in bulk and in tablet dosage form. For the determina...

A simple spectrophotometric method was developed for the determination of captopril (CPL) in pharmaceutical preparations. The method is based on coupling captopril with 2,6-dichloroquinone-4-chlorimide (DCQ) in dimethylsulphoxide. The yellow reaction product was measured at 443 nm. The absorbance-concentration plot was rectilinear over the range of 10-50 μg/mL with minimum detection limit (LOD)...

Two simple, sensitive, specific, spectrophotometric methods have been developed for the determination of ceftaroline fosamil in bulk and Injection forms. The λmax of ceftaroline fosamil was found to be 245.2 nm in distilled water. The zero order UV Spectrophotometric method exhibits high sensitivity, with linearity and obeys beer’s law in the range of 2 – 10 μg/mL. The limit of detection and qu...

The objective of the current study was to determine tablet content and perform dissolution test of expired tablets and tablets which expiry date has not exceeded. The analyzed tablets contained metoprolol tartrate (50 mg) and propranolol hydrochloride (10 mg), respectively. Content determination was performed using spectrophotometric method with UV detection; the percent of dissolved substance ...