Occupational contact with livestock is an established risk factor for exposure to livestock-associated methicillin-resistant Staphylococcus aureus (MRSA), particularly among industrial swine workers. While S. aureus is known to infect cattle, livestock-associated S. aureus carriage among workers in the beef production chain has received limited attention. Beefpacking workers, who slaughter, but...

Three hundred and twenty isolates of Staphylococcus aureus were typed by DNA sequence analysis of the X region of the protein A gene (spa). spa typing was compared to both phenotypic and molecular techniques for the ability to differentiate and categorize S. aureus strains into groups that correlate with epidemiological information. Two previously characterized study populations were examined. ...

$EVWUDFW (IILFLHQW LQWHURUJDQL]DWLRQDO EXVLQHVV SURFHVVHV LQ WKH ILHOG RI H FRPPHUFH UHTXLUH WKH LQWHJUDWLRQ RI HOHFWURQLF GRFXPHQW DQG GDWD LQWHUFKDQJH DQG LQWHURUJDQL]DWLRQDO SURFHVVHV :H SURSRVH WR VXSSRUW LQWHURUJDQL]DWLRQDO EXVLQHVV SURFHVVHV E\ VR FDOOHG ;0/ QHWV D QHZ NLQG RI KLJK OHYHO 3HWUL QHWV ;0/ QHWV DUH D IRUPDO JUDSKLFDO PRGHOLQJ ODQJXDJH WKDW DOORZV WR PRGHO ERWK WKH IORZ RI ;0/...

BACKGROUND In the spondyloarthropathies, the underlying molecular and cellular pathways driving disease are poorly understood. By undertaking a study in knee synovial biopsies from spondyloarthropathy (SpA) and ankylosing spondylitis (AS) patients we aimed to elucidate dysregulated genes and pathways. METHODS RNA was extracted from six SpA, two AS, three osteoarthritis (OA) and four normal co...

INTRODUCTION Methicillin-resistant Staphylococcus aureus (MRSA) is globally recognized as an important public health problem. Whereas comprehensive molecular typing data of MRSA strains is available, particularly in Europe, North America and Australia, similar information is very limited in sub-Saharan Africa including Zambia. METHODS In this study, thirty two clinical isolates of Staphylococ...

Selectively-bred obesity-resistant [diet resistant (DR)] rats weigh less than obesity-prone [diet-induced obese (DIO)] rats, despite comparable daily caloric intake, suggesting phenotypic energy expenditure differences. Human data suggest that obesity is maintained by reduced ambulatory or spontaneous physical activity (SPA). The neuropeptide orexin A robustly stimulates SPA. We hypothesized th...

Recombinant N-myristoyltransferase of Plasmodium falciparum (termed PfNMT) has been used in the development of a SPA (scintillation proximity assay) suitable for automation and high-throughput screening of inhibitors against this enzyme. The ability to use the SPA has been facilitated by development of an expression and purification system which yields considerably improved quantities of solubl...

We develop an approach for identifying groups or families of Staphylococcus aureus bacteria based on genotype data. With the emergence of drug resistant strains, S. aureus represents a significant human health threat. Identifying the family types efficiently and quickly is crucial in community settings. Here, we develop a hybrid sequence algorithm approach to type this bacteria using only its s...