coronary  atherosclerotic  disease  is  one  of  the  most  endangering  health  disorder worldwide. this study was designed to investigate the correlation between hla-drβ1 alleles and circulating th1/th2 type cytokines in coronary atherosclerosis. by elisa, th1/th2 type cytokines were determined in serum samples of 31 subjects with unstable angina, 27 subjects with chronic stable angina and 24...

chronic myeloid leukemia (cml) is a myeloproliferative disease of the hematopoietic stem cells, characterized by the presence of the philadelphia (ph) chromosome. although imatinib inhibits the bcr-abl kinase activity, clinical experiences confirm that imatinib may not target cml stem cells in vivo. the identification of signaling pathways responsible for the self-renewal properties of leukemic...

helicobacter pylori was the main cause of gastric cancer (gc) in all over the world that results in intestinal- and diffuse-type carcinoma. infection by this bacterium remains chronic for a long time. in vitro studies have highlighted the role of stem cells in gc. h. pylori infection results in tissue injury and the adult/tissue stem cells subsequently start to replace the dead cells. the genet...

background:   objective: this study presents an economical and simple co-culture system for pluripotent stem cells generation from neonatal mouse testis   materials and methods: isolated testicular cells were cultured in dmem/f12. characteristics of the isolated cells and obtained es-like cell were immune-cytochemically confirmed by examining the presence of plzf, vimentin, oct4 and nanog prote...

conclusions these outcomes support our theory that rapamycin increases oxidative stress in mcf-7 and mda mb-231 cells but also shows high levels of antioxidant effects, which probably limit the effects of the rapamycin on the same issue in the clinic. methods the mcf-7 and mda mb-231 cell lines were cultivated and treated with rapamycin for 72 hours. the viability of the cells was determined us...

there are reports from different countries that some types of glaucoma are associated with blood groups. this cross-sectional study was performed on 400 glaucomatous patients [100 patients in each group of primary open angle glaucoma (poag), chronic angle closure glaucoma (cacg), pseudoexfoliative glaucoma (pexg) and primary congenital glaucoma (pcg)] and 400 blood donors as control group to as...

objective: the aim of the present study was to investigate the effect of protein and dna components of toxoplasma gondii on maturation of dendritic cells and their efficiency in il-12 production and proliferation of t cells. materials and methods: for dc generation, bone marrow cells were cultured in the presence of gm-csf and il-4 for 5 days. tumor lysate and protein or dna components of toxo...

background: rna interference-based gene silencing has recently been applied as an efficient tool for functional gene analysis. rorc2 is the key transcription factor orchestrating th17 cells differentiation, the cells that are known as the pathogenic elements in various autoimmune diseases. the aim of this study was to design efficient sirnas specific for rorc2 and to evaluate different criteria...

objective: nanosilver has numerous applications in medicine due to its potent antibacterial activity. however, data regarding the bio-safety of its effective concentrations is scarce. this study aims to assess the toxicity of silver nanoparticles on human gingival epithelial cells under in-vitro conditions.   methods: this in vitro study evaluated the toxic effects of filtered and unfiltered na...

twik2 ion channels (k2p6.1, encoded by a gene named kcnk6) belong to the family of two-pore domain potassium channels. twik2 is also considered as a “silent” channel because of its inability to produce measurable currents in heterologous expression systems and its intracellular retention. it is expressed in different mammalian tissues including lymphoid organs and the vascular system. however, ...