Cancer metastasis involves multistep adhesive interactions between tumor cells (TCs) and endothelial cells (ECs), but the molecular mechanisms of intercellular communication in the tumor microenvironment remain elusive. Using static and flow coculture systems in conjunction with flow cytometry, we discovered that certain receptors on the ECs are upregulated on melanoma cell adhesion. Direct con...

OBJECTIVES The aim of this study was to elucidate the role of IL-8 for neutrophil recruitment in nonallergic CRS patients. METHODS After coculture of Streptococcus pneumoniae (SP) with the mucosal epithelial cells (MECs) from non-CRS patients, at three different SP/MEC (1/1, 10/1, 100/1) ratios, the expression of IL-8 mRNA and the concentration of IL-8 were measured by RT-PCR and ELISA. The e...

BACKGROUND/AIMS Skin biopsies allow for direct phenotyping of the endothelium in clinical settings. OBJECTIVES We hypothesize that in murine sepsis endothelial activation is manifested by changes in protein and mRNA expression in skin biopsies, and that such alterations differ from other organs. METHODS In two mouse models of sepsis [endotoxemia and cecal ligation puncture (CLP)], we measur...

The hot water extract of Rabdosia rubescens was traditionally used as an antithrombotic medicine. To explore its antithrombotic utility and mechanism, we carried out a series of in vitro and in vivo assays in this study. In vitro platelet aggregation assay showed that the half maximal inhibitory concentration values of aqueous extract of R. rubescens leaves (AERL) inhibiting platelet aggregatio...

normal 0 false false false en-us x-none fa background : circulating endothelial cells (cecs) and e-selectin are known as sensitive and specific markers of endothelial dysfunction. this study investigated whether cecs and e-selectin are surrogate biomarkers of preeclampsia and if measurement of cecs and e-selectin, early in the third trimester, could be a means of predicting preeclampsia. method...

BACKGROUND Gestational thrombocytopenia is more likely to occur in twin than singleton pregnancies. However, it is unclear whether platelets are more reactive in twin than singleton pregnancies. METHODS Changes in spontaneous platelet aggregation and concomitant fall in platelet count were examined over 90min after blood sampling in 171 and 52 citrated whole blood (CWB) samples from 59 and 17...

Systemic treatment with bevacizumab is associated with increased rates of arterial and venous thromboembolism and haemorrhage. In order to investigate the pathophysiological mechanism involved, platelet adhesive and aggregatory functions were tested with a platelet function analyser (PFA-100®) in an in vitro study and in a longitudinal clinical observation study. For the in vitro study, blood f...

Background. We investigated the level of soluble adhesion molecules in diabetic patients and the effect of the peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist rosiglitazone on plasma levels of adhesion molecules and an inflammation marker in type 2 diabetic patients with coronary artery disease (CAD) after percutaneous coronary intervention (PCI). Methods. A total of 116 d...

Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by loss of tolerance toward self-nucleic acids, autoantibody production, interferon expression and signaling, defect in the regulatory T (T reg ) cell compartment. In this work, we identified that platelets from patients with active SLE preferentially interacted cells via P-selectin/P-selectin glycoprotein ligand-...