Phosphorus nuclear magnetic resonance (3’P NMR) is an increasingly important physical technique for the elucidation of structural features of phospholipid bilayer membranes [l-S] . In sonicated vesicles, however, the chemical shift differences between different classes of phospholipids are of approximately the same magnitude as the widths of the resonances themselves [5,6]. For many application...

Viable leucocytes obtained fresh from normal human subjects were shown to be able to catalyse the in vitro iodination of bovine serum albumin (BSA) in a H2O2-generating system. The rate and degree of iodination were greatly improved by sonication of the cells. A balanced salt solution was a more favourable medium than phosphate buffer for the myeloperoxidase (MPO)-catalysed iodination of whole ...

Dilute ( ~10% w/v) unsonicated phosphatidylserine dispersions in Hz0 contain spherical particles of a wide size distribution (0.1 to 15 q). The majority have diameters between 0.1 to 2 pm and lack the multilamellar arrangement of bilayers typical of unsonicated lecithin dispersions. Sonication uroduces small thermodynamically unstable vesicles surrounded by a single closed bilayer with an avera...

1. Distearoyl phosphatidylcholine and the phosphonium analogue, in which the nitrogen atom is replaced by phosphorus, show similar gel-liquid crystalline transition temperatures as detected by differential scanning calorimetry. 2. The temperature-dependence of the 31P n.m.r. (nuclear-magnetic-resonance) linewidths of the phosphate resonances of sonicated vesicles of distearoyl phosphatidylcholi...

In this study the IgG subclass antibodies to sonicated preparations of Mycobacterium leprae (leprosin A) and BCG (BCG-S) as well as to purified recombinant 65 kDa protein of M. leprae (rML65) were analysed in sera from leprosy patients and healthy household contacts (HFC) and noncontacts (HNC) in a leprosy endemic population. In LBI+ (lepromatous bacterial index positive) patients, IgG3 was pre...

Phosphoenolpyruvate carboxykinase (adenosine 5'-triphosphate) was the only enzyme capable of carboxylating pyruvate or phosphoenolpyruvate that could be demonstrated in sonicated cells or cell-free extracts of a group 1 butyrivibrio.

In this study, the effects of adding graphene nanoplatelets (GPLs) prepared at different probe sonication times such as 1, 2, 4 and 6 h on microstructure mechanical properties silicon carbide (SiC) ceramic were investigated. Scanning electron microscopy (SEM) examinations size measurements revealed that dimension GPLs decreased with increasing time. However, reduction in was very low up to 2 ti...

Chlamydomonas reinhardtii cells disrupted under low pressure in a Yeda press yielded a preparation ("presate") with high permeability toward substrates for Class A chloroplasts and intact mitochondria. The stoichiometric rates of CO2 uptake and O2 photoevolution by the wild-type cell pressate were severely suppressed by 10 mM exogenous phosphate, and this suppression could be reversed by the ad...