Somatic hybridization can be an interesting alternative for the selection of heterozygous and vigorous potato plants through combination of dihaploid genomes. The resulting hybrids can harbour interesting characters and thus can be used in agriculture if they are in agreement with agronomic criteria. In this report, we used an intraspecific somatic hybridization technique for the production of ...

Source/Description: cpX12 is a 1.1 kb EcoRI single-copy genomic fragment cloned into pAT153 from a pool of 100 cosmids isolated from a human/hamster somatic cell hybrid containing only the human X chromosome (1). Polymorphism: cpX12 reveals a two-allele restriction fragment length polymorphism with bands at 0.7 kb and 1.9 kb (Al) or 2.6 kb (A2) after Southern blot analysis of human genomic DNA ...

Source/Description: NB84 is a 1.0 kb Hindm-EcoRI fragment isolated from two pooled chromosome 22 specific libraries (AT-CC # 57733 and ATCC # 57714) and is subcloned into pUC9. Polymorphism: PstI digestion of genomic DNA and hybridization with the probe detects a two allele polymorphism: 4.6 kb (Al) and 4.0 kb (A2). No constant bands were present. Chromosomal Localization: Regional localization...

Source/Description: NB17 is a 0.7 kb HindlO-EcoRI fragment isolated from two pooled chromosome 22 specific libraries (AT-CC # 57733 and ATCC # 57714) and was subcloned into pUC9. Polymorphism: Bgin or TaqI digestion of genomic DNA and hybridization with the probe detects a two allele polymorphism: Bgin (Al: 12.5 kb, A2: 4.0 kb) with a constant band at 3.0 kb; TaqI (Bl: 2.9 kb, B2: 2.2 kb). The ...

plant tissue culture is an alternative method of commercial propagation and is being used widely for the commercial propagation of a large number of plant species, including many medicinal plants. somatic embryogenesis is a process by which asexual or somatic cells are induced to form embryos in culture. somatic embryogenesis is a multi-step regeneration process starting with formation of pro-e...

Fluorescence in situ hybridization (FISH) is the most direct method for physically mapping DNA sequences on chromosomes. Fluorescence in situ hybridization mapping of meiotic chromosomes during the pachytene stage is an important tool in plant cytogenetics, because it provides high-resolution measurements of physical distances. Fluorescence in situ hybridization mapping of coffee pachytene chro...

Introduction A number of immunoglobulin (Ig) secreting human hybrid cell lines were created using one-on-one somatic cell hybridization of a rare human tumor infiltrating B lymphocyte and a cell of a novel human cell line (WTM), developed in house and described earlier [1]. These hybrid cell lines secret various amounts of tumor-derived immunoglobulins (Igs) of different specificities. Current ...

Global genomic analysis of transposable element distributions of both natural (En/Spm, Ac-Ds, and MuDR/Mu) and modified (RescueMu) types was performed by fluorescence in situ hybridization (FISH) on somatic chromosomes coupled with karyotyping of each chromosome. In lines without an active transposable element, the locations of silent En/Spm, Ac-Ds, and MuDR/Mu elements were visualized, reveali...

The technique of somatic cell fusion has made it possible to study cell biology in an unusual and direct way. When cells are mixed in the presence of Sendai virus, their membranes coalesce, the cytoplasm becomes intermingled, and multinucleated homoand heterokaryons are formed by fusion of similar or different cells, respectively (1, 2). By fusing cells which contrast in some important biologic...