Many plant cells can be reprogrammed into a pluripotent state that allows ectopic organ development. Inducing totipotent states to stimulate somatic embryo (SE) development is, however, challenging due to insufficient understanding of molecular barriers that prevent somatic cell dedifferentiation. Here we show that Polycomb repressive complex 2 (PRC2)-activity imposes a barrier to hormone-media...

The green flagellate J’olvox is a simple muhicellular organism having only two kinds of cells, somatic and reproductive. The cells are arranged on the periphery of a spheroid. Asexual individu~s of I’olvox carten’ are composed of 2000-4000 somatic cells and about 16 large reproductive cells (gonidia), located in the posterior region of the spheroid. Mature asexual spheroids show a very regular ...

Somatic cell nuclear transfer (SCNT) is a reproductive biotechnology with great potential in the reproduction of different species zootechnical interest, including sheep. This study aimed to carry out bibliometric analysis scientific papers published on application SCNT sheep during period 1997–2023. The search involved Science Citation Index Expanded and Social Sciences databases main collecti...

The focus of the current project was to establish somatic embryogenesis protocols for the tropical pine species Pinus oocarpa using immature zygotic embryos (ZEs) as explants. Somatic embryogenesis is best supported by mimicking natural seed-embryo developmental conditions, through a tissue culture medium formulation based on the mineral content of the seed nutritive tissue [megagametophyte (MG...

Increasing infertility is one of the most serious health problems today. Over past few years, we have had opportunity to follow progress technologies focused on production embryos in vitro (i.e., fertilization and intracytoplasmic sperm injection, genetic engineering, or somatic cell nuclear transfer. Oocyte maturation important processes vitro. Despite recent this field, developmental competen...

The effect of 2, 4-D and TDZ concentrations on primary embryogenesis seem to be genotype dependent. There were significant differences between the control and the other treatments on somatic embryo production by genotype AMAZ 3-2. Genotype COCA 3370-5 did not show any significant difference among the treatments with 1mg/l 2, 4-D + 1μg/l TDZ, 1mg/l 2,4-D + 10μg/l TDZ and the control treatment. T...

Somatic embryogenesis is an example of induced cellular totipotency, where embryos develop from vegetative cells rather than from gamete fusion. Somatic embryogenesis can be induced in vitro by exposing explants to growth regulators and/or stress treatments. The BABY BOOM (BBM) and LEAFY COTYLEDON1 (LEC1) and LEC2 transcription factors are key regulators of plant cell totipotency, as ectopic ov...

Somatic embryogenesis is an efficient micro-propagation technique, which can lead to mass multiplication of tea with a tap root. Hence, the development viable somatic and protocol through liquid culture conditions needed increase efficiency protocol. Cotyledon-derived embryos, leaf stem callus TRI 5001 cultivar, were tested different MS media. A significantly higher cotyledon-derived embryo rat...