Green manure could improve soil nutrients and crop production, playing a significant role in sustainable agriculture. However, the impacts of green manure on crop health and the roles soil microbial communities play in the process haven’t been clarified clearly yet. In this study, we investigated soil microbial community composition and structure in four tobacco farmlands, which were treated wi...

We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide solution. This method was used to construct large-insert soil metagenomic librar...

In this study microorganisms from Antarctic soils were isolated using different selective nutrient media. The soils were collected from different habitats of Livingston Island. The microorganisms were isolated and purified to pure culture. The isolated microorganisms were micro-morphologically and macromorphologically characterized. The results obtained suggested that despite the extreme Antarc...

the focus of this study was antimicrobial membrane-activity of actinomycetes isolated from soils of iran. in this work, soil samples were collected from desert and farming zones of northern and central iran. a total number of 45 actinomycetes were isolated from the soil samples. in the primary screening performed to evaluate antimicrobial activity, isolated microorganisms were analyzed in terms...

Soil microbial diversity is an important index of agricultural productivity (Nimisha Sharma et al,.2010; Kent and Triplett, 2002; Isik, 2008). Both plant and the soil types influences the microbial diversity. Rhizosphere microbial diversity usually influenced by both plant and soil types. Plants and microorganisms interact with each other and co evaluate together. Their balance is important for...

Demand of all living organisms on the same nutrients forms the basis for interspecific competition between plants and microorganisms in soils. This competition is especially strong in the rhizosphere. To evaluate competitive and mutualistic interactions between plants and microorganisms and to analyse ecological consequences of these interactions, we analysed 424 data pairs from 41 (15)N-labell...

Biodegradation of diesel oil (5 g(middot)kg [soil dry weight](sup-1)) was investigated in five alpine subsoils, differing in soil type and bedrock, in laboratory experiments during 20 days at 10(deg)C. The biodegradation activities of the indigenous soil microorganisms and of a psychrotrophic diesel oil-degrading inoculum and the effect of biostimulation by inorganic fertilization (C/N/P ratio ...

Microorganisms decompose herbicides and they may serve as bioindicators of soil changes following herbicide application. Certain microbial species may be used as bioherbicides. This study has shown that Azotobacter is most sensitive to herbicide application; it is, therefore, a reliable indicator of the biological value of soil. The numbers of this group of nitrogen-fixing bacteria decrease con...

Plants interact with a diversity of microorganisms, and there is often concordance in their community structures. Because most community-level studies are observational, it is unclear if such concordance arises because of host specificity, in which microorganisms or plants limit each other's occurrence. Using a reciprocal transplant experiment, we tested the hypothesis that host specificity bet...

Four greenhouse studies were conducted to evaluate the effects of native prairie grasses and two pesticide-degrading bacteria to remediate atrazine and metolachlor in soils from agricultural dealerships (Alpha site soil, northwest Iowa, USA; Bravo site soil, central Iowa, USA). The Alpha soil contained a low population of atrazine-degrading microorganisms relative to the Bravo soil. Each soil f...