The salt-induced formation of duplex structure (primarily hairpin loops) in denatured calf thymus DNA was monitored by measuring the decrease in absorbance at 260 nm as a function of increasing sodium ion concentration. It was found that this process was noncooperative and could be accurately described by the mass-action expression for the reversible formation of a binary complex: single strand...

This work introduces a polymer-free optode nanosensor for ratiometric sodium imaging. Transmembrane ion dynamics are often captured by electrophysiology and calcium imaging, but sodium dyes suffer from short excitation wavelengths and poor selectivity. Optodes, optical sensors composed of a polymer matrix with embedded sensing chemistry, have been translated into nanosensors that selectively im...

An ion exchange-gravimetric method, previously developed as a National Committee for Clinical Laboratory Standards (NCCLS) reference method for the determination of sodium in human serum, has been re-evaluated and improved. Sources of analytical error in this method have been examined more critically and the overall uncertainties decreased. Additionally, greater accuracy and repeatability have ...

The basis for the reduced maximum diastolic potential of canine cardiac subendocardial Purkinje fibers surviving one day after extensive transmural infarction was investigated, using double-barrel potassium and sodium ion-sensitive microelectrodes. The maximum diastolic potential of Purkinje fibers in infarct preparations from the left ventricular apex measured during the first hour of superfus...

Some neuronal receptors perceive external input in the form of hybrid periodic signals. The signal detection may be based on the mechanism of vibrational resonance, in which a system's response to the low frequency signal can become optimal by an appropriate choice of the vibration amplitude of HFS. The vibrational resonance effect is investigated in a neuron model in which the intra- and extra...

channelopathies were known, but most of these drugs were used empirically and were found to act on ion channels afterward. Now our knowledge about their role in diseases have validated ion channels as very promising druggable targets. What are the difficulties encountered in ion channel drug discovery? A major problem of ion channel drugs regards the side effects. An ion channel type is often e...

Kidney collecting duct principal cells play a key role in regulated tubular reabsorption of water and sodium and secretion of potassium. The importance of this function for the maintenance of the osmotic homeostasis of the whole organism motivates extensive study of the ion transport properties of collecting duct principal cells. We performed experimental measurements of cell volume and intrace...

[1] A three-dimensional Monte Carlo model of Mercury’s neutral sodium exosphere was used to describe correlation between the observed variations in the exospheric density and the variations in the surface concentration of sodium along Mercury’s motion around the Sun [Leblanc and Johnson, 2003]. Four processes of ejection were taken into account: thermal and photon stimulated desorptions, solar ...

Tightly regulated ion homeostasis throughout the body is necessary for the prevention of such debilitating states as dehydration.(1) In contrast, rapid ion fluxes at the cellular level are required for initiating action potentials in excitable cells.(2) Sodium regulation plays an important role in both of these cases; however, no method currently exists for continuously monitoring sodium levels...

The apparent distribution coefficients, Kapp, of some sugar alcohols to cation-exchange resins in the sodium-ion form and with divinylbenzene (DVB) contents 2, 4, 6, 8% were measured at 25ºC. Assuming that binding constant, B, ribitol sodium ion was zero, swelling pressures, Π, DVB 8％ estimated be 3.2, 9.4, 12.3, 25.7 MPa, respectively. From these Π values Kapp values, B each alcohol calculated...