Natural colorants have been widely used in food and cosmetic products. Nonionic and anionic surfactants are also usually added as solubilizers and stabilizers in the products. Surfactants form a special structure, a so-called micelle. Micelles can alter stabilities and physicochemical properties of other chemicals in the system. The objectives of this study were to determine stabilities in mice...

Antiserum against starfish egg myosin was produced in rabbits. Antibody specificity to myosin was demonstrated by Ouchterlony's immunodiffusion test and by immunoelectrophoresis in the presence of sodium dodecylsulfate (SDS). The latter technique showed that the antibody binds to both heavy and light chains of egg myosin. Furthermore, the antibody reacted with starfish sperm mysosin and starfis...

In previous studies we found that anionic surfactants such as sodium laurate (SL) and/or sodium dodecylsulfate (SDS) exert actions on epidermal keratinocytes rather than mast cells to give rise of histamine production and skin itching through increasing the expression of the 53-kDa active form of L-histidine decarboxylase (HDC). In addition, with treatment of SL in a three-dimensional human ker...

Cell aggregation is a stress response and serves as a survival strategy for Pseudomonas aeruginosa strain PAO1 during growth with the toxic detergent Na-dodecylsulfate (SDS). This process involves the psl operon and is linked to c-di-GMP signalling. The induction of cell aggregation in response to SDS was studied. Transposon and site-directed mutagenesis revealed that the cupA-operon and the co...

The presented contribution is aimed on the study of influence of various surfactants and polymers on the kinetics of reduction of diamminsilver complex cation in alkaline media using D-maltose as a reducing sugar (modified Tollens reaction). From the group of surfactants, the following types were used: anionic (sodium dodecylsulfate and sodium dodecylbenzenesulfonate), cationic (cetyltrimethyla...

Separation of twelve sympathomimetic amines and related compounds by micellar electrokinetic chromatography (MEKC) with UV absorbance detection is described. These amines were well separated within 25 min using 50 mM sodium tetraborate solution containing 15 mM sodium dodecylsulfate (SDS) of pH 9.3 as a running solution and detected at 210 nm. MEKC was performed with an applied voltage of 13 kV...

Sodium/D-glucose cotransport carriers solubilized from intestinal brush border membranes were purified and incorporated into liposomes made of soybean phospholipids, and transport properties of the reconstituted system were studied. The brush border membrane vesicles prepared from guinea pig small intestine were first treated with deoxycholate and papain in order to remove unnecessary membrane ...

Electron paramagnetic resonance (EPR) has been used to monitor the interaction of bovine serum albumin (BSA) with cationic cethyltrimethylammonium chloride (CTAC) at pH 7.0. EPR results using 5-DSA and 16-DSA nitroxide spin labels show that in the presence of BSA the EPR spectra are composed of two label populations, one contacting the protein and a second one due to label localization in the m...

In this work, the naturally occurring -hairpin antimicrobial peptide protegrin-1 (PG-1) is studied by molecular dynamics simulation in all-atom sodium dodecylsulfate and dodecylphosphocholine micelles. These simulations provide a high-resolution picture of the interactions between the peptide and simple models of bacterial and mammalian membranes. Both micelles show significant disruption, as i...