Abbreviations: ABA, abscisic acid; BSA, bovine serum albumin; CAP, micropylar area around the radicle emergence point or endosperm cap; DAB, diaminobenzidine; DEAE, diethylaminoethyl; IgG, immunoglobulin G; LAT, lateral coat plus endosperm; LBG, locust bean gum; ηsp, specific viscosity; PBS, phosphate buffer saline; pI, isoelectric point; RAD, radicle tip; SDS-PAGE, sodium dodecyl sulfate polya...

The synthesis of the murein precursor lipid I is performed by MraY. We have shown that mraY is an essential gene for cell growth. Cells depleted of MraY first swell and then lyse. The expression of mraY DNA in vitro produces a 40-kDa polypeptide detectable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Isoelectric focusing of avian tumor viruses with distinct type-specific envelope antigens demonstrated no differences in isoelectric points. Viruses with different type-specific antigens were found to contain different glycoprotein components when virion proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

A cytopathic factor was isolated from M. hyopneumoniae VPP11 membranes. This factor had an isoelectric point of 6.2 and was capable of inducing cytopathic effect in MRC-5 human lung fibroblast culture at a concentration of 250 ng of protein per ml. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of this cytopathic factor showed a major band with a molecular weight of approximately 54,...

Outer membrane proteins (OMPs) expressed by Vibrio tubiashii under different environmental growth conditions were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and PCR analyses. Results showed the presence of a 38- to 40-kDa OmpU-like protein and ompU gene, a maltoporin-like protein, several novel OMPs, and a regulatory toxR homolog.

DNase activities in different protein fractions of Frankia strain R43 were studied. The extracellular and the cell wall-associated fractions revealed the presence of exo- and endonucleolytic enzymes, but none was detected in the cytoplasmic fraction. The strongest DNase hydrolysis was found in the extracellular fraction, in which six DNases were detected by sodium dodecyl sulfate-polyacrylamide...

We have identified a yeast protein that resembles actins from other eucaryotes in its tight binding to pancreatic deoxyribonuclease I, its copolymerizaton with purified muscle actin, its one-dimensional peptide map, and its apparent polymerization into 7-nm filaments. The yeast actin-like protein yielded a single spot on two-dimensional polyacrylamide gel electrophoresis, suggesting that a sing...

Our objectives were to determine if milk casein as a percentage of true protein (CN%TP) estimated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) is equivalent CN%TP Kjeldahl, and the proportion (CN), proteolysis products (CNPP), serum (SP) from (TP) that goes into Kjeldahl noncasein nitrogen (NCN) filtrate stays in NCN precipitate using SDS-PAGE. Raw samples collected 1...