background: free-living amoebae have various genera that are found in several environmental niches such as soil, freshwater, dust, seawater and hot springs. most of free-living amoebae are normally harmless to humans. however, some such as acanthamoeba and also naegleria fowleri , balamuthia manderillaris and sappinia are identified as opportunistic and pathogenic amoebae that can cause eye dis...